TM Antibody (D-3): sc-13164

TM Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects TM protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). TM (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thrombomodulin (TM), also known as CD141, is a type I membrane receptor predominantly found on endothelial cells, where TM plays a crucial role in coagulation regulation. TM features a cysteine-rich extracellular domain with six EGF-like regions, which are essential for TM′s interaction with thrombin. This interaction activates protein C, leading to activated protein C (APC) generation, an important anticoagulant enzyme that, with protein S, inhibits coagulation by inactivating factors Va and VIIIa. TM′s significance in maintaining hemostatic balance is underscored by TM gene deletion resulting in embryonic lethality in mice, highlighting TM′s essential role in vascular biology and coagulation homeostasis.

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TM Antibody (D-3) References:

1. Human thrombomodulin gene is intron depleted: nucleic acid sequences of the cDNA and gene predict protein structure and suggest sites of regulatory control.  |  Jackman, RW., et al. 1987. Proc Natl Acad Sci U S A. 84: 6425-9. PMID: 2819876
2. Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.  |  Suzuki, K., et al. 1987. EMBO J. 6: 1891-7. PMID: 2820710
3. Gene structure of human thrombomodulin, a cofactor for thrombin-catalyzed activation of protein C.  |  Shirai, T., et al. 1988. J Biochem. 103: 281-5. PMID: 2836377
4. Thrombomodulin Regulation of Mitogen-Activated Protein Kinases.  |  Giri, H., et al. 2019. Int J Mol Sci. 20: PMID: 30991642
5. Thrombomodulin, a Master Switch Controlling Poststroke Microvascular Remodeling and Angiogenesis.  |  Hermann, DM. and Kleinschnitz, C. 2020. Arterioscler Thromb Vasc Biol. 40: 2818-2820. PMID: 33232207
6. Thrombomodulin is upregulated in the kidneys of women with pre-eclampsia.  |  van Aanhold, CCL., et al. 2021. Sci Rep. 11: 5692. PMID: 33707524
7. Chondrocyte Thrombomodulin Protects against Osteoarthritis.  |  Kang, L., et al. 2023. Int J Mol Sci. 24: PMID: 37298473
8. Absence of the blood-clotting regulator thrombomodulin causes embryonic lethality in mice before development of a functional cardiovascular system.  |  Healy, AM., et al. 1995. Proc Natl Acad Sci U S A. 92: 850-4. PMID: 7846065
9. The absence of the blood clotting regulator thrombomodulin causes embryonic lethality in mice before development of a functional cardiovascular system.  |  Rosenberg, RD. 1995. Thromb Haemost. 74: 52-7. PMID: 8578517
10. Mutation of protease domain residues Lys37-39 in human protein C inhibits activation by the thrombomodulin-thrombin complex without affecting activation by free thrombin.  |  Gerlitz, B. and Grinnell, BW. 1996. J Biol Chem. 271: 22285-8. PMID: 8798383
11. The Gla26 residue of protein C is required for the binding of protein C to thrombomodulin and endothelial cell protein C receptor, but not to protein S and factor Va.  |  Nishioka, J., et al. 1996. Thromb Haemost. 75: 275-82. PMID: 8815576
12. Factor V and protein S as cofactors to activated protein C.  |  Dahlbäck, B. 1997. Haematologica. 82: 91-5. PMID: 9107093

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TM Antibody (D-3) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-TM Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TM Antibody (D-3) Specifications

Alternate Product Name: Thrombomodulin

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 22-321

Epitope Target: TM

Epitope Species: human

Product Reactivity/Detection: thrombomodulin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for TM Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TM antibody is TM Antibody (H-11): sc-271804.