TLR7 Antibody (4F4) recommended for detection of TLR7 of human origin by WB, IP and IF

TLR7 Antibody (4F4): sc-57463

TLR7 Antibody (4F4) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-TLR7 Antibody (4F4) is a mouse monoclonal IgG1 (kappa light chain) TLR7 antibody provided at 50 µg/0.5 ml
    • raised against amino acids 451-500 of TLR7 of human origin
    • recommended for detection of TLR7 of human origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR7 (4F4): sc-57463.
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Seria possível dizerem-me qual a sequência do imunógeno?

Asked by: PinhoP
Thank you for your question. This antibody was raised against amino acids 451-500 of TLR7 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2020-02-05

I am using TLR7 (4F4): sc-57463 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from Zeigt leider eine unspezifische Bande An mit verschiedenen TLRs stabil transduzierten HEK-Zell-Kulturen getestet. Zeigt leider in allen Lysaten eine Bande, nicht nur bei TLR7.
Date published: 2018-05-30
Rated 5 out of 5 by from Gute Ergebnisse Immunfluoreszenz Habe verschiedene Tumor-Zelllinien getestet und der AK hat super funktioniert.
Date published: 2017-12-15
Rated 5 out of 5 by from We found the product working extremely well We found the product working extremely well on cervical tissue and would recommend others working on human tissue to use the antibody.
Date published: 2015-11-11
Rated 3 out of 5 by from Signal via western blot analysis of TLR7 Signal via western blot analysis of TLR7 expression in Ramos whole cell lysate. -SCBT QC
Date published: 2015-02-13
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