TLK1 Antibody (S-32): sc-100345

TLK1 Antibody (S-32) is a mouse monoclonal IgG3 antibody that detects TLK1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TLK1, a member of the Tousled-like kinase family, plays a crucial role in cell cycle regulation and is essential for proper organ morphogenesis, particularly during the development of multicellular organisms. TLK1 is predominantly localized in the nucleus, where TLK1 is involved in critical processes such as DNA replication and repair. Nuclear localization of TLK1 is vital as TLK1 interacts with substrates involved in these processes, ensuring genomic stability and proper cell division. Notably, TLK1 exhibits a propensity to dimerize through its coiled-coil structure, which enhances kinase activity and ability to autophosphorylate, further amplifying regulatory functions during the S phase of the cell cycle. Expression of TLK1 is tightly regulated, with peak activity observed during DNA synthesis, highlighting TLK1′s importance in spermatogenesis and overall cellular development. With versatile applications and significant biological role, anti-TLK1 antibody (S-32) is an invaluable tool for researchers studying cell cycle dynamics and developmental biology.

The preferred TLK1 antibody is TLK1 Antibody (G-8): sc-393515.