Tim44 Antibody (A-9): sc-390755
- Tim44 Antibody (A-9) is a mouse monoclonal IgG1 κ Tim44 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 144-400 mapping within an internal region of Tim44 of human origin
- Tim44 Antibody (A-9) is recommended for detection of Tim44 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Tim44 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Tim44 Antibody (A-9) for WB applications. These reagents are now offered in bundles with Tim44 Antibody (A-9) (see ordering information below).
Tim44 Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tim44 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim44 (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tim44 protein plays a crucial role in mitochondrial protein import, specifically in the translocation of nuclear-encoded preproteins into the mitochondrial matrix. Tim44 functions as a key component of the translocase of the inner mitochondrial membrane (TIM) complex, which is essential for proper functioning of mitochondria, the powerhouse of the cell. Tim44 interacts with mitochondrial heat shock protein 70 (Hsp70), facilitating the transfer of preproteins from the TIM channel into the mitochondrial matrix. This interaction is vital for maintaining mitochondrial function and cellular energy production, as it ensures necessary proteins are correctly imported and folded within mitochondria. Tim44′s importance in mitochondrial biogenesis and function highlights its potential as a target for studying mitochondrial-related diseases and disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tim44 Antibody (A-9) References:
- Genetic and structural characterization of the human mitochondrial inner membrane translocase. | Bauer, MF., et al. 1999. J Mol Biol. 289: 69-82. PMID: 10339406
- Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein. | Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
- The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. | Moro, F., et al. 1999. EMBO J. 18: 3667-75. PMID: 10393182
- A multisubunit complex of outer and inner mitochondrial membrane protein translocases stabilized in vivo by translocation intermediates. | Schülke, N., et al. 1999. J Biol Chem. 274: 22847-54. PMID: 10428870
- The transport machinery for the import of preproteins across the outer mitochondrial membrane. | Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
- Protein import into mitochondria. | Neupert, W. 1997. Annu Rev Biochem. 66: 863-917. PMID: 9242927
- Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria. | Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tim44 Antibody (A-9) | sc-390755 | 200 µg/ml | $322.00 | |||
Tim44 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-530251 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tim44 Antibody (A-9): m-IgGκ BP-HRP Bundle | sc-523668 | 200 µg Ab, 40 µg BP | $361.00 | |||
Tim44 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-543958 | 200 µg Ab; 20 µg BP | $361.00 | |||
Tim44 Antibody (A-9) AC | sc-390755 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tim44 Antibody (A-9) HRP | sc-390755 HRP | 200 µg/ml | $322.00 | |||
Tim44 Antibody (A-9) FITC | sc-390755 FITC | 200 µg/ml | $336.00 | |||
Tim44 Antibody (A-9) PE | sc-390755 PE | 200 µg/ml | $349.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 488 | sc-390755 AF488 | 200 µg/ml | $364.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 546 | sc-390755 AF546 | 200 µg/ml | $364.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 594 | sc-390755 AF594 | 200 µg/ml | $364.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 647 | sc-390755 AF647 | 200 µg/ml | $364.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 680 | sc-390755 AF680 | 200 µg/ml | $364.00 | |||
Tim44 Antibody (A-9) Alexa Fluor® 790 | sc-390755 AF790 | 200 µg/ml | $364.00 |