Tim23 Antibody (H-8): sc-514463

Tim23 Antibody (H-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Tim23 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tim23 (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tim23 plays a crucial role in the translocation of nuclear-encoded preproteins into the mitochondrial matrix, a process essential for mitochondrial function and cellular energy production. Located in the inner mitochondrial membrane, Tim23 is part of the translocase of the inner membrane (Tim) complex, which also includes Tim17 and Tim44. These proteins work together to facilitate the import of preproteins, ensuring proper integration into the mitochondria where they can perform their functions. Tim23 and its interacting partners are vital for maintaining mitochondrial integrity and function, as disruptions in this process can lead to mitochondrial diseases and impaired cellular metabolism. The presence of Tim23 in both fetal and adult tissues underscores its importance in cellular physiology, making anti-Tim23 antibody (H-8) an invaluable tool for researchers studying mitochondrial dynamics and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tim23 Antibody (H-8) References:

1. Genetic and structural characterization of the human mitochondrial inner membrane translocase.  |  Bauer, MF., et al. 1999. J Mol Biol. 289: 69-82. PMID: 10339406
2. Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein.  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
3. The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix.  |  Moro, F., et al. 1999. EMBO J. 18: 3667-75. PMID: 10393182
4. A multisubunit complex of outer and inner mitochondrial membrane protein translocases stabilized in vivo by translocation intermediates.  |  Schülke, N., et al. 1999. J Biol Chem. 274: 22847-54. PMID: 10428870
5. The transport machinery for the import of preproteins across the outer mitochondrial membrane.  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
6. From TOM to the TIM23 complex - handing over of a precursor.  |  Callegari, S., et al. 2020. Biol Chem. 401: 709-721. PMID: 32074073
7. TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.  |  Akabane, S., et al. 2023. Cell Rep. 42: 112454. PMID: 37160114
8. Structural basis of mitochondrial protein import by the TIM23 complex.  |  Sim, SI., et al. 2023. Nature. 621: 620-626. PMID: 37344598
9. Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex.  |  Zhou, X., et al. 2023. Nat Struct Mol Biol. 30: 1996-2008. PMID: 37696957
10. TOM-TIM23 supercomplex formation.  |  Jain, N., et al. 2024. Methods Enzymol. 707: 3-22. PMID: 39488380
11. Protein import into mitochondria.  |  Neupert, W. 1997. Annu Rev Biochem. 66: 863-917. PMID: 9242927
12. Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tim23 Antibody (H-8) has 72 citations in a variety of scientific publications.

Primary antibodies like Anti-Tim23 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tim23 Antibody (H-8) Specifications

Alternate Product Name: Tim23

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 31-209

Epitope Target: Tim23

Epitope Species: human

Product Reactivity/Detection: Tim23

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 72 citations in various scientific publications

Available Conjugates/Forms for Tim23 Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin