TIGAR Antibody (E-2): sc-166290

TIGAR Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects TIGAR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TIGAR (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIGAR protein, also known as TP53 (tumor protein 53)-induced glycolysis and apoptosis regulator or C12orf5, is a 270 amino acid protein that plays a crucial role in cellular metabolism and stress response. Induced by the p53 tumor suppressor pathway, TIGAR functions to mitigate oxidative stress by modulating glycolytic flux. Specifically, TIGAR shares sequence similarity with the bisphosphate domain of fructose-2,6-bisphosphate degrading enzyme, allowing TIGAR to lower intracellular levels of fructose-2,6-bisphosphate. By inhibiting glycolysis, TIGAR redirects metabolic pathways toward the pentose phosphate shunt, which is vital for generating NADPH and ribose-5-phosphate, essential for nucleotide synthesis and cellular antioxidant defense. This mechanism underscores TIGAR′s importance in protecting cells from reactive oxygen species, which can cause DNA damage and trigger apoptosis, thereby highlighting TIGAR′s potential role in cancer biology and therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TIGAR Antibody (E-2) References:

1. Hereditary pancreatitis: a model for inflammatory diseases of the pancreas.  |  Schneider, A. and Whitcomb, DC. 2002. Best Pract Res Clin Gastroenterol. 16: 347-63. PMID: 12079262
2. Identification of novel p53 target genes in ionizing radiation response.  |  Jen, KY. and Cheung, VG. 2005. Cancer Res. 65: 7666-73. PMID: 16140933
3. p53 and metabolism: Inside the TIGAR.  |  Green, DR. and Chipuk, JE. 2006. Cell. 126: 30-2. PMID: 16839873
4. TIGAR, a p53-inducible regulator of glycolysis and apoptosis.  |  Bensaad, K., et al. 2006. Cell. 126: 107-20. PMID: 16839880
5. The regulation of energy generating metabolic pathways by p53.  |  Corcoran, CA., et al. 2006. Cancer Biol Ther. 5: 1610-3. PMID: 17204863
6. CFTR gene mutations in pancreatitis: Frequency and clinical manifestations in an Austrian patient cohort.  |  Zoller, H., et al. 2007. Wien Klin Wochenschr. 119: 527-33. PMID: 17943404
7. The histidine phosphatase superfamily: structure and function.  |  Rigden, DJ. 2008. Biochem J. 409: 333-48. PMID: 18092946
8. TIGAR mediates the inhibitory role of hypoxia on ROS production and apoptosis in rat nucleus pulposus cells.  |  Jiang, LB., et al. 2018. Osteoarthritis Cartilage. 26: 138-148. PMID: 29061494
9. TIGAR Attenuates High Glucose-Induced Neuronal Apoptosis via an Autophagy Pathway.  |  Zhou, W., et al. 2019. Front Mol Neurosci. 12: 193. PMID: 31456661
10. TIGAR Promotes Tumorigenesis and Protects Tumor Cells From Oxidative and Metabolic Stresses in Gastric Cancer.  |  Liu, Z., et al. 2019. Front Oncol. 9: 1258. PMID: 31799200
11. Activation of p53-regulated pro-survival signals and hypoxia-independent mitochondrial targeting of TIGAR by human papillomavirus E6 oncoproteins.  |  Yapindi, L., et al. 2023. Virology. 585: 1-20. PMID: 37257253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIGAR Antibody (E-2) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-TIGAR Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIGAR Antibody (E-2) Specifications

Alternate Product Name: TIGAR

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 61-269

Epitope Target: TIGAR

Epitope Species: mouse

Product Reactivity/Detection: TIGAR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for TIGAR Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin