TIGAR Antibody (E-10): sc-166291
- TIGAR Antibody (E-10) is a mouse monoclonal IgG2b κ, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 61-269 mapping at the C-terminus of TIGAR of mouse origin
- Anti-TIGAR Antibody (E-10) is recommended for detection of TIGAR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TIGAR Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TIGAR Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with TIGAR Antibody (E-10) (see ordering information below).
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SEE ALSO...
TIGAR Antibody (E-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects TIGAR in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TIGAR (E-10) antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. TIGAR, also known as TP53-induced glycolysis and apoptosis regulator or C12orf5, is a 270 amino acid protein that plays a crucial role in cellular metabolism and stress response. TIGAR is induced by the p53 tumor suppressor pathway, which is vital for maintaining genomic stability and preventing tumorigenesis. TIGAR functions by modulating the glycolytic pathway; TIGAR shares sequence similarity with the fructose-2,6-bisphosphate degrading enzyme, allowing TIGAR to lower intracellular levels of fructose-2,6-bisphosphate. This action effectively inhibits glycolysis and redirects metabolic flux towards the pentose phosphate pathway, which is essential for generating NADPH and ribose-5-phosphate. By decreasing the concentration of reactive oxygen species, TIGAR helps protect cells from oxidative stress, which can lead to DNA damage and apoptosis. TIGAR′s ability to regulate these metabolic processes underscores its importance in cellular defense mechanisms and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TIGAR Antibody (E-10) References:
- Knockdown of TIGAR by RNA interference induces apoptosis and autophagy in HepG2 hepatocellular carcinoma cells. | Ye, L., et al. 2013. Biochem Biophys Res Commun. 437: 300-6. PMID: 23817040
- Identification of TP53-induced glycolysis and apoptosis regulator (TIGAR) as the phosphoglycolate-independent 2,3-bisphosphoglycerate phosphatase. | Gerin, I., et al. 2014. Biochem J. 458: 439-48. PMID: 24423178
- TIGAR has a dual role in cancer cell survival through regulating apoptosis and autophagy. | Xie, JM., et al. 2014. Cancer Res. 74: 5127-38. PMID: 25085248
- TIGAR knockdown radiosensitizes TrxR1-overexpressing glioma in vitro and in vivo via inhibiting Trx1 nuclear transport. | Zhang, Y., et al. 2017. Sci Rep. 7: 42928. PMID: 28338004
- Loss of TIGAR Induces Oxidative Stress and Meiotic Defects in Oocytes from Obese Mice. | Wang, H., et al. 2018. Mol Cell Proteomics. 17: 1354-1364. PMID: 29776966
- TIGAR suppresses seizures induced by kainic acid through inhibiting oxidative stress and neuronal apoptosis. | Chen, C., et al. 2019. Biochem Biophys Res Commun. 515: 436-441. PMID: 31160088
- Expression of TIGAR and its correlation with clinicopathology, prognosis, and 18F-FDG PET/CT parameters in patients with resectable pancreatic ductal adenocarcinoma. | Song, Y., et al. 2021. Nucl Med Commun. 42: 528-534. PMID: 33481504
- Decitabine Downregulates TIGAR to Induce Apoptosis and Autophagy in Myeloid Leukemia Cells. | Li, L., et al. 2021. Oxid Med Cell Longev. 2021: 8877460. PMID: 33532040
- Overexpression of TIGAR and HO-1 in peripheral blood mononuclear cells (PBMCs) of breast cancer patients treated with radiotherapy. | Dianati-Nasab, A., et al. 2022. Int J Radiat Biol. 98: 1551-1558. PMID: 35452587
- High Glucose Activates Prolyl Hydroxylases and Disrupts HIF-α Signaling via the P53/TIGAR Pathway in Cardiomyocyte. | Chen, JX., et al. 2023. Cells. 12: PMID: 37048134
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIGAR Antibody (E-10) | sc-166291 | 200 µg/ml | $322.00 | |||
TIGAR Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-521558 | 200 µg Ab, 40 µg BP | $361.00 | |||
TIGAR Antibody (E-10): m-IgG2b BP-HRP Bundle | sc-548926 | 200 µg Ab; 10 µg BP | $361.00 | |||
TIGAR Antibody (E-10) AC | sc-166291 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TIGAR Antibody (E-10) HRP | sc-166291 HRP | 200 µg/ml | $322.00 | |||
TIGAR Antibody (E-10) FITC | sc-166291 FITC | 200 µg/ml | $336.00 | |||
TIGAR Antibody (E-10) PE | sc-166291 PE | 200 µg/ml | $349.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 488 | sc-166291 AF488 | 200 µg/ml | $364.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 546 | sc-166291 AF546 | 200 µg/ml | $364.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 594 | sc-166291 AF594 | 200 µg/ml | $364.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 647 | sc-166291 AF647 | 200 µg/ml | $364.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 680 | sc-166291 AF680 | 200 µg/ml | $364.00 | |||
TIGAR Antibody (E-10) Alexa Fluor® 790 | sc-166291 AF790 | 200 µg/ml | $364.00 |