TIF1β Antibody (D-7): sc-515790

TIF1β Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects TIF1β protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TIF1β Antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIF1β protein, also known as transcription intermediary factor 1-beta, KRAB-associated protein 1, and tripartite motif-containing 28, plays a crucial role in transcriptional regulation by interacting with the Kruppel-associated box (KRAB) repression domain found in numerous transcription factors. This interaction is vital for gene silencing, as TIF1β recruits chromatin remodeling complexes and histone-modifying enzymes to target genes, thereby influencing cellular processes such as differentiation, development, and response to stress. The human TIF1β gene is located on chromosome 19q13.4 and encodes an 835 amino acid nuclear protein characterized by three zinc-binding domains, a RING finger, B-boxes, and a coiled-coil domain, which are essential for transcriptional repression. The presence of both a Cys/His PHD finger and a bromodomain in TIF1β further enhances transcriptional control capabilities, making TIF1β a significant player in gene expression regulation across various biological contexts.

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TIF1β Antibody (D-7) References:

1. Two novel Krüppel-associated box-containing zinc-finger proteins, KRAZ1 and KRAZ2, repress transcription through functional interaction with the corepressor KAP-1 (TIF1beta/KRIP-1).  |  Agata, Y., et al. 1999. J Biol Chem. 274: 16412-22. PMID: 10347202
2. Sequence-specific transcriptional repression by KS1, a multiple-zinc-finger-Krüppel-associated box protein.  |  Gebelein, B. and Urrutia, R. 2001. Mol Cell Biol. 21: 928-39. PMID: 11154279
3. Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.  |  Schultz, DC., et al. 2001. Genes Dev. 15: 428-43. PMID: 11230151
4. Germ cell expression of the transcriptional co-repressor TIF1beta is required for the maintenance of spermatogenesis in the mouse.  |  Weber, P., et al. 2002. Development. 129: 2329-37. PMID: 11973266
5. Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites.  |  Bártová, E., et al. 2007. Histochem Cell Biol. 127: 375-88. PMID: 17205308
6. Phosphorylation at Ser473 regulates heterochromatin protein 1 binding and corepressor function of TIF1beta/KAP1.  |  Chang, CW., et al. 2008. BMC Mol Biol. 9: 61. PMID: 18590578
7. TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.  |  Seki, Y., et al. 2010. Proc Natl Acad Sci U S A. 107: 10926-31. PMID: 20508149
8. TIF1β association with HP1 is essential for post-gastrulation development, but not for Sertoli cell functions during spermatogenesis.  |  Herzog, M., et al. 2011. Dev Biol. 350: 548-58. PMID: 21163256
9. TIF1β is phosphorylated at serine 473 in colorectal tumor cells through p38 mitogen-activated protein kinase as an oxidative defense mechanism.  |  Shen, LT., et al. 2017. Biochem Biophys Res Commun. 492: 310-315. PMID: 28864417
10. KAP-1, a novel corepressor for the highly conserved KRAB repression domain.  |  Friedman, JR., et al. 1996. Genes Dev. 10: 2067-78. PMID: 8769649
11. Transcriptional repression by RING finger protein TIF1 beta that interacts with the KRAB repressor domain of KOX1.  |  Moosmann, P., et al. 1996. Nucleic Acids Res. 24: 4859-67. PMID: 9016654
12. Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression.  |  Chang, CJ., et al. 1998. Mol Cell Biol. 18: 5880-7. PMID: 9742105

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIF1β Antibody (D-7) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TIF1β Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIF1β Antibody (D-7) Specifications

Alternate Product Name: TRIM28

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 536-835

Epitope Target: TIF1β

Epitope Species: human

Product Reactivity/Detection: TIF1β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TIF1β Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent