TIAR Antibody (G-6): sc-398372

TIAR Antibody (G-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects TIAR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TIAR (G-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. TIAR protein, also known as TIA1 cytotoxic granule-associated RNA binding protein like 1, plays a crucial role in regulating RNA metabolism and is involved in cellular response to stress, particularly in apoptosis. Nuclear localization allows TIAR to interact with pre-mRNA and influence splicing and stability, thereby impacting gene expression and cellular function. TIAR expression occurs in a wide range of cell types, particularly those of hematopoietic origin, underscoring importance in various physiological and pathological processes, including immune responses and cell death pathways. TIAR′s relationship with TIA-1 protein highlights RNA-binding proteins′ complexity in regulating cellular fate, making anti-TIAR antibody (G-6) an essential tool for studying these critical biological processes.

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TIAR Antibody (G-6) References:

1. Translational repression by RNA-binding protein TIAR.  |  Mazan-Mamczarz, K., et al. 2006. Mol Cell Biol. 26: 2716-27. PMID: 16537914
2. Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR.  |  Izquierdo, JM. 2006. Biochem Biophys Res Commun. 348: 703-11. PMID: 16890199
3. Fas-activated serine/threonine kinase (FAST K) synergizes with TIA-1/TIAR proteins to regulate Fas alternative splicing.  |  Izquierdo, JM. and Valcárcel, J. 2007. J Biol Chem. 282: 1539-43. PMID: 17135269
4. Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein.  |  Izquierdo, JM. and Valcárcel, J. 2007. J Biol Chem. 282: 19410-7. PMID: 17488725
5. Effects of infliximab therapy on gene expression levels of tumor necrosis factor alpha, tristetraprolin, T cell intracellular antigen 1, and Hu antigen R in patients with rheumatoid arthritis.  |  Sugihara, M., et al. 2007. Arthritis Rheum. 56: 2160-9. PMID: 17599736
6. Bombyx mori nucleopolyhedrovirus nucleic acid binding proteins BRO-B and BRO-E associate with host T-cell intracellular antigen 1 homologue BmTRN-1 to influence protein synthesis during infection.  |  Kotani, E., et al. 2015. J Gen Virol. 96: 1947-56. PMID: 25834094
7. Fas and its ligand in a general mechanism of T-cell-mediated cytotoxicity.  |  Hanabuchi, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 4930-4. PMID: 7515183
8. The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death.  |  Taupin, JL., et al. 1995. Proc Natl Acad Sci U S A. 92: 1629-33. PMID: 7533298
9. Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis.  |  Tian, Q., et al. 1995. J Exp Med. 182: 865-74. PMID: 7544399
10. A revertant TCR gamma delta + cell clone which has lost MHC nonrestricted cytotoxic activity but retains redirected killing upon stimulation of the CD3 receptor.  |  Visonneau, S., et al. 1995. Cell Immunol. 165: 252-65. PMID: 7553890
11. TIA-1: structural and functional studies on a new class of cytolytic effector molecule.  |  Anderson, P. 1995. Curr Top Microbiol Immunol. 198: 131-43. PMID: 7774278
12. Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.  |  Dember, LM., et al. 1996. J Biol Chem. 271: 2783-8. PMID: 8576255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIAR Antibody (G-6) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-TIAR Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIAR Antibody (G-6) Specifications

Alternate Product Name: TIAL1

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 348-377

Epitope Target: TIAR

Epitope Species: human

Product Reactivity/Detection: TIAR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for TIAR Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred TIA-1/TIAR antibody is TIA-1/TIAR Antibody (D-9): sc-48371.