Thrombospondin 1 Antibody (SPM321): sc-65612
- Thrombospondin 1 Antibody (SPM321) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
- raised against reduced and alkylated purified Thrombospondin 1 (fully denatured) from the supernatant of Thrombin-activated platelets of human origin
- recommended for detection of reduced and non-reduced Thrombospondin 1 of mouse, rat, human, ovine, equine, bovine, porcine and canine origin by WB, IP, IF and IHC(P)
- See Thrombospondin 1 (C-8): sc-393504 for Thrombospondin 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Thrombospondin 1 Antibody (SPM321) for WB and IHC(P) applications. These reagents are now offered in bundles with Thrombospondin 1 Antibody (SPM321) (see ordering information below).
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Thrombospondin 1 Antibody (SPM321) is a mouse monoclonal IgG1 kappa light chain antibody specifically engineered to recognize Thrombospondin 1 (TSP-1), also known as CD36 ligand or cartilage-derived retinoic acid-sensitive protein (CD-RAP) in cartilage-related studies. Anti-Thrombospondin 1 antibody (SPM321) is raised against reduced and alkylated purified human Thrombospondin 1, ensuring robust recognition of both reduced and fully denatured forms. Thrombospondin 1 is a multifunctional extracellular glycoprotein that undergoes critical post-translational modifications, including extensive glycosylation, which are essential for stability, secretion, and interactions with cell surface receptors such as CD36 and integrins. These post-translational modifications enable TSP-1 to regulate key biological processes such as platelet aggregation, cellular adhesion, migration, and tissue remodeling, making TSP-1 a vital component in both physiological and pathological contexts. Thrombospondin 1 (SPM321) antibody demonstrates broad species reactivity, effectively detecting TSP-1 from mouse, rat, human, ovine, equine, bovine, porcine, and canine origins across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). By targeting post-translationally modified forms of TSP-1, anti-Thrombospondin 1 antibody (SPM321) allows researchers to investigate functional implications of these modifications in cellular processes and disease mechanisms. Thrombospondin 1 (SPM321) monoclonal antibody is an indispensable tool for advancing studies in cellular biology, regenerative medicine, and oncology, providing critical insights into dynamic roles of TSP-1 in maintaining cellular homeostasis and mediating tissue responses to injury.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thrombospondin 1 Antibody (SPM321) References:
- Thrombospondin-1: a physiological regulator of nitric oxide signaling. | Isenberg, JS., et al. 2008. Cell Mol Life Sci. 65: 728-42. PMID: 18193160
- Thrombospondin-1: an extracellular message delivered by macrophages that promotes aortic aneurysms. | Lessey-Morillon, EC. and Roberts, DD. 2015. Circ Res. 117: 113-5. PMID: 26139855
- Original insights on thrombospondin-1-related antireceptor strategies in cancer. | Jeanne, A., et al. 2015. Front Pharmacol. 6: 252. PMID: 26578962
- Role of thrombospondin 1 in liver diseases. | Li, Y., et al. 2017. Hepatol Res. 47: 186-193. PMID: 27492250
- Thrombospondin-1, Free Radicals, and the Coronary Microcirculation: The Aging Conundrum. | LeBlanc, AJ. and Kelm, NQ. 2017. Antioxid Redox Signal. 27: 785-801. PMID: 28762749
- Thrombospondin-1 interactions regulate eicosanoid metabolism and signaling in cancer-related inflammation. | Ramirez, MU., et al. 2018. Cancer Metastasis Rev. 37: 469-476. PMID: 29909440
- Thrombospondin-1 at the crossroads of corpus luteum fate decisions. | Farberov, S., et al. 2019. Reproduction. 157: R73-R83. PMID: 30566900
- Thrombospondin-1 Restricts Interleukin-36γ-Mediated Neutrophilic Inflammation during Pseudomonas aeruginosa Pulmonary Infection. | Peñaloza, HF., et al. 2021. mBio. 12: PMID: 33824208
- Dual Role of Thrombospondin-1 in Flow-Induced Remodeling. | Grenier, C., et al. 2021. Int J Mol Sci. 22: PMID: 34769516
- Thrombospondin-1 is an endogenous substrate of cereblon responsible for immunomodulatory drug-induced thromboembolism. | Hatakeyama, K., et al. 2024. Blood Adv. 8: 785-796. PMID: 38163319
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thrombospondin 1 Antibody (SPM321) | sc-65612 | 200 µg/ml | $322.00 | |||
Thrombospondin 1 Antibody (SPM321): m-IgG Fc BP-HRP Bundle | sc-539185 | 200 µg Ab; 10 µg BP | $361.00 | |||
Thrombospondin 1 Antibody (SPM321): m-IgG1 BP-HRP Bundle | sc-541315 | 200 µg Ab; 20 µg BP | $361.00 |