THAP7 Antibody (F-7): sc-515496
- THAP7 Antibody (F-7) is a mouse monoclonal IgG1 κ THAP7 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 40-65 within an internal region of THAP7 of human origin
- THAP7 Antibody (F-7) is recommended for detection of THAP7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-THAP7 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-515496 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for THAP7 Antibody (F-7) for WB applications. These reagents are now offered in bundles with THAP7 Antibody (F-7) (see ordering information below).
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THAP7 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects THAP7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). THAP7 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. THAP7 protein, also known as THAP domain-containing protein 7, consists of 309 amino acids and is a member of the Thanatos-associated protein family, which plays a crucial role in gene regulation and chromatin remodeling. THAP7 is predominantly expressed in the testis and is found throughout the cell, particularly in the nucleus, where THAP7 interacts with chromatin and preferentially binds to hypoacetylated histones H3 and H4 via its C-terminal tail. This interaction allows THAP7 to repress transcription by recruiting histone deacetylase 3 and nuclear receptor co-repressor to specific promoters, leading to the deacetylation of histone H3 and ultimately influencing gene expression. The gene encoding human THAP7 is located on chromosome 22q11.2, highlighting its importance in the context of genetic regulation and potential implications in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
THAP7 Antibody (F-7) References:
- The THAP domain: a novel protein motif with similarity to the DNA-binding domain of P element transposase. | Roussigne, M., et al. 2003. Trends Biochem Sci. 28: 66-9. PMID: 12575992
- N-CoR-HDAC corepressor complexes: roles in transcriptional regulation by nuclear hormone receptors. | Jones, PL. and Shi, YB. 2003. Curr Top Microbiol Immunol. 274: 237-68. PMID: 12596910
- Human THAP7 is a chromatin-associated, histone tail-binding protein that represses transcription via recruitment of HDAC3 and nuclear hormone receptor corepressor. | Macfarlan, T., et al. 2005. J Biol Chem. 280: 7346-58. PMID: 15561719
- The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity. | Clouaire, T., et al. 2005. Proc Natl Acad Sci U S A. 102: 6907-12. PMID: 15863623
- Thanatos-associated protein 7 associates with template activating factor-Ibeta and inhibits histone acetylation to repress transcription. | Macfarlan, T., et al. 2006. Mol Endocrinol. 20: 335-47. PMID: 16195249
- Disruption of HDAC4/N-CoR complex by histone deacetylase inhibitors leads to inhibition of IL-2 gene expression. | Matsuoka, H., et al. 2007. Biochem Pharmacol. 74: 465-76. PMID: 17559812
- The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing. | Miele, A., et al. 2007. J Cell Biochem. 102: 136-48. PMID: 17577209
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THAP7 Antibody (F-7) | sc-515496 | 200 µg/ml | $322.00 | |||
THAP7 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-531394 | 200 µg Ab; 10 µg BP | $361.00 | |||
THAP7 Antibody (F-7): m-IgGκ BP-HRP Bundle | sc-525072 | 200 µg Ab, 40 µg BP | $361.00 | |||
THAP7 Antibody (F-7): m-IgG1 BP-HRP Bundle | sc-544525 | 200 µg Ab; 20 µg BP | $361.00 | |||
THAP7 Antibody (F-7) X | sc-515496 X | 200 µg/0.1 ml | $322.00 | |||
THAP7 Antibody (F-7) AC | sc-515496 AC | 500 µg/ml, 25% agarose | $424.00 | |||
THAP7 Antibody (F-7) HRP | sc-515496 HRP | 200 µg/ml | $322.00 | |||
THAP7 Antibody (F-7) FITC | sc-515496 FITC | 200 µg/ml | $336.00 | |||
THAP7 Antibody (F-7) PE | sc-515496 PE | 200 µg/ml | $349.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 488 | sc-515496 AF488 | 200 µg/ml | $364.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 546 | sc-515496 AF546 | 200 µg/ml | $364.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 594 | sc-515496 AF594 | 200 µg/ml | $364.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 647 | sc-515496 AF647 | 200 µg/ml | $364.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 680 | sc-515496 AF680 | 200 µg/ml | $364.00 | |||
THAP7 Antibody (F-7) Alexa Fluor® 790 | sc-515496 AF790 | 200 µg/ml | $364.00 | |||
THAP7 (F-7) Neutralizing Peptide | sc-515496 P | 100 µg/0.5 ml | $69.00 |