TGFβ3 Antibody (G-9): sc-166833
- TGFβ3 Antibody (G-9) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 350-375 at the C-terminus of TGFβ3 of human origin
- recommended for detection of precursor and mature TGFβ3 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, porcine, canine and avian
- Anti-TGF beta 3 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TGFβ3 Antibody (G-9) for WB and IHC(P) applications. These reagents are now offered in bundles with TGFβ3 Antibody (G-9) (see ordering information below).
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TGFβ3 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects TGFβ3 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). TGFβ3 (G-9) antibody is available in non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The transforming growth factor beta (TGFβ) family, including TGFβ1, TGFβ2, and TGFβ3, regulates cellular processes such as proliferation, differentiation, and apoptosis. TGFβ3 is essential for proper embryonic development and tissue homeostasis, mediating intercellular signaling that influences cell fate decisions during development. TGFβ3 is synthesized as a precursor protein, cleaved to produce a 112 amino acid polypeptide, which remains associated with a latent portion of the molecule, highlighting complex regulation and function. TGFβ3 expression levels are significant in various pathological conditions, showing overexpression in extramammary Paget′s disease and downregulation in Bowen′s disease, making TGFβ3 a valuable biomarker for tumor activity. TGFβ3 levels correlate strongly with insulin-like growth factor 1 and osteocalcin levels in serum, suggesting involvement in bone metabolism and potential implications in bone-related diseases. Glucocorticoids can inhibit TGFβ3 production by modulating mRNA levels and c-Jun activity, indicating a regulatory mechanism that could be targeted for therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TGFβ3 Antibody (G-9) References:
- Human leiomyoma smooth muscle cells show increased expression of transforming growth factor-beta 3 (TGF beta 3) and altered responses to the antiproliferative effects of TGF beta. | Lee, BS. and Nowak, RA. 2001. J Clin Endocrinol Metab. 86: 913-20. PMID: 11158066
- Molecular cloning and characterization of the human and porcine transforming growth factor-beta type III receptors. | Morén, A., et al. 1992. Biochem Biophys Res Commun. 189: 356-62. PMID: 1333192
- Regulatory mutations in transforming growth factor-beta3 gene cause arrhythmogenic right ventricular cardiomyopathy type 1. | Beffagna, G., et al. 2005. Cardiovasc Res. 65: 366-73. PMID: 15639475
- A large-scale genetic association study of ossification of the posterior longitudinal ligament of the spine. | Horikoshi, T., et al. 2006. Hum Genet. 119: 611-6. PMID: 16609882
- Transforming growth factor-beta. A family of growth regulatory peptides. | Miller, DA., et al. 1990. Ann N Y Acad Sci. 593: 208-17. PMID: 2375595
- TGFβ2 and TGFβ3 isoforms drive fibrotic disease pathogenesis. | Sun, T., et al. 2021. Sci Transl Med. 13: PMID: 34349032
- Utilizing PK and PD Biomarkers to Guide the First-in-Human Starting Dose Selection of MTBT1466A: A Novel Humanized Monoclonal Anti-TGFβ3 Antibody for the Treatment of Fibrotic Diseases. | Yadav, R., et al. 2023. J Pharm Sci. 112: 2910-2920. PMID: 37429356
- Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. | Derynck, R., et al. Nature. 316: 701-5. PMID: 3861940
- Genome-wide in vivo CRISPR screen identifies TGFβ3 as actionable biomarker of palbociclib resistance in triple negative breast cancer. | Poulet, S., et al. 2024. Mol Cancer. 23: 118. PMID: 38831405
- Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. | Todaro, GJ., et al. 1980. Proc Natl Acad Sci U S A. 77: 5258-62. PMID: 6254071
- Sarcoma growth factor from conditioned medium of virally transformed cells is composed of both type alpha and type beta transforming growth factors. | Anzano, MA., et al. 1983. Proc Natl Acad Sci U S A. 80: 6264-8. PMID: 6604914
- Abnormal lung development and cleft palate in mice lacking TGF-beta 3 indicates defects of epithelial-mesenchymal interaction. | Kaartinen, V., et al. 1995. Nat Genet. 11: 415-21. PMID: 7493022
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGFβ3 Antibody (G-9) | sc-166833 | 200 µg/ml | $322.00 | |||
TGFβ3 Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-529064 | 200 µg Ab; 10 µg BP | $361.00 | |||
TGFβ3 Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-521763 | 200 µg Ab, 40 µg BP | $361.00 | |||
TGFβ3 Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-543297 | 200 µg Ab; 20 µg BP | $361.00 | |||
TGFβ3 Antibody (G-9) AC | sc-166833 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TGFβ3 Antibody (G-9) HRP | sc-166833 HRP | 200 µg/ml | $322.00 | |||
TGFβ3 Antibody (G-9) FITC | sc-166833 FITC | 200 µg/ml | $336.00 | |||
TGFβ3 Antibody (G-9) PE | sc-166833 PE | 200 µg/ml | $349.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 488 | sc-166833 AF488 | 200 µg/ml | $364.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 546 | sc-166833 AF546 | 200 µg/ml | $364.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 594 | sc-166833 AF594 | 200 µg/ml | $364.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 647 | sc-166833 AF647 | 200 µg/ml | $364.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 680 | sc-166833 AF680 | 200 µg/ml | $364.00 | |||
TGFβ3 Antibody (G-9) Alexa Fluor® 790 | sc-166833 AF790 | 200 µg/ml | $364.00 | |||
TGFβ3 (G-9) Neutralizing Peptide | sc-166833 P | 100 µg/0.5 ml | $69.00 |