TFIIH p89 Antibody (B-7): sc-377301

TFIIH p89 Antibody (B-7) is a mouse monoclonal IgG2b antibody that detects TFIIH p89 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIH p89, also known as XPB or ERCC3, plays a crucial role in the initiation of transcription and DNA repair, making TFIIH p89 essential for maintaining genomic stability and proper gene expression. TFIIH p89 is part of the larger TFIIH complex, which is located in the nucleus and is involved in unwinding DNA during transcription and nucleotide excision repair. The presence of ATP-dependent helicase activity in TFIIH p89 allows TFIIH p89 to separate the DNA strands, facilitating the recruitment of other transcription factors and RNA polymerase II. Additionally, the interaction of TFIIH p89 with other subunits, such as p80 (XPD or ERCC2), enhances TFIIH p89′s functionality in both transcription and DNA repair processes. The ability of TFIIH p89 to coordinate these critical cellular functions underscores TFIIH p89′s importance in cellular health and the prevention of diseases associated with DNA damage, such as cancer.

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TFIIH p89 Antibody (B-7) References:

1. The TFIIH subunit p89 (XPB) localizes to the centrosome during mitosis.  |  Weber, A., et al. 2010. Cell Oncol. 32: 121-30. PMID: 20208140
2. XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase.  |  Fuss, JO. and Tainer, JA. 2011. DNA Repair (Amst). 10: 697-713. PMID: 21571596
3. XPB helicase regulates DNA incision by the Thermoplasma acidophilum endonuclease Bax1.  |  Roth, HM., et al. 2012. DNA Repair (Amst). 11: 286-93. PMID: 22237014
4. XPB: An unconventional SF2 DNA helicase.  |  Fan, L. and DuPrez, KT. 2015. Prog Biophys Mol Biol. 117: 174-181. PMID: 25641424
5. Spironolactone-induced degradation of the TFIIH core complex XPB subunit suppresses NF-κB and AP-1 signalling.  |  Elinoff, JM., et al. 2018. Cardiovasc Res. 114: 65-76. PMID: 29036418
6. The XPB Subunit of the TFIIH Complex Plays a Critical Role in HIV-1 Transcription and XPB Inhibition by Spironolactone Prevents HIV-1 Reactivation from Latency.  |  Mori, L., et al. 2021. J Virol. 95: PMID: 33239456
7. Comprehensive analysis of ERCC3 prognosis value and ceRNA network in AML.  |  Bao, X., et al. 2023. Clin Transl Oncol. 25: 1053-1066. PMID: 36472749
8. Relationship between XPA, XPB/ERCC3, XPF/ERCC4, and XPG/ERCC5 Polymorphisms and the Susceptibility to Head and Neck Carcinoma: A Systematic Review, Meta-Analysis, and Trial Sequential Analysis.  |  Imani, MM., et al. 2024. Medicina (Kaunas). 60: PMID: 38541204
9. Proteomic Ligandability Maps of Spirocycle Acrylamide Stereoprobes Identify Covalent ERCC3 Degraders.  |  Liu, Z., et al. 2024. J Am Chem Soc. 146: 10393-10406. PMID: 38569115
10. A mutation in the XPB/ERCC3 DNA repair transcription gene, associated with trichothiodystrophy.  |  Weeda, G., et al. 1997. Am J Hum Genet. 60: 320-9. PMID: 9012405

The preferred TFIIH p89 antibody is TFIIH p89 Antibody (G-10): sc-271500.