TFIIH p89 Antibody (G-10): sc-271500

TFIIH p89 Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIH p89 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIH p89 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIH p89, also known as XPB or ERCC3, plays a crucial role in transcription and DNA repair mechanisms, functioning as a helicase that unwinds DNA. This unwinding is essential for proper transcription of protein-coding genes, as it allows RNA polymerase II and other transcription factors to access DNA template. Additionally, TFIIH is involved in nucleotide excision repair, a critical pathway for maintaining genomic integrity by repairing bulky DNA lesions caused by environmental factors such as UV radiation. The complex nature of TFIIH, which includes multiple subunits with distinct functions, underscores its importance in cellular processes, making anti-TFIIH p89 antibody (G-10) a valuable tool for researchers studying transcription regulation and DNA repair pathways.

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TFIIH p89 Antibody (G-10) References:

1. Cloning of the 62-kilodalton component of basic transcription factor BTF2.  |  Fischer, L., et al. 1992. Science. 257: 1392-5. PMID: 1529339
2. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.  |  Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
3. Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.  |  Gerard, M., et al. 1991. J Biol Chem. 266: 20940-5. PMID: 1939143
4. The TFIIH subunit p89 (XPB) localizes to the centrosome during mitosis.  |  Weber, A., et al. 2010. Cell Oncol. 32: 121-30. PMID: 20208140
5. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.  |  Weeda, G., et al. 1990. Cell. 62: 777-91. PMID: 2167179
6. ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.  |  Weber, CA., et al. 1990. EMBO J. 9: 1437-47. PMID: 2184031
7. Protein predictive signatures for lymph node metastasis of gastric cancer.  |  Li, W., et al. 2013. Int J Cancer. 132: 1851-9. PMID: 23011604
8. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
9. Protein Profiling of Bladder Urothelial Cell Carcinoma.  |  Hu, J., et al. 2016. PLoS One. 11: e0161922. PMID: 27626805
10. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.  |  Humbert, S., et al. 1994. EMBO J. 13: 2393-8. PMID: 8194529
11. Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH.  |  Marinoni, JC., et al. 1997. EMBO J. 16: 1093-102. PMID: 9118947
12. Expression of DNA excision-repair-cross-complementing proteins p80 and p89 in brain of patients with Down Syndrome and Alzheimer's disease.  |  Hermon, M., et al. 1998. Neurosci Lett. 251: 45-8. PMID: 9714461

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIH p89 Antibody (G-10) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIH p89 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIH p89 Antibody (G-10) Specifications

Alternate Product Name: ERCC3

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 764-782

Epitope Target: TFIIH p89

Epitope Species: human

Product Reactivity/Detection: TFIIH p89

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for TFIIH p89 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent