TFIIH p62 Antibody (H-10): sc-25329

TFIIH p62 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIH p62 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-TFIIH p62 antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIH p62 plays a crucial role in the nucleotide excision repair pathway, which is essential for maintaining genomic stability by repairing DNA damage caused by ultraviolet light and other environmental factors. TFIIH p62 is part of the larger TFIIH complex, which not only facilitates transcription initiation by RNA polymerase II but also possesses helicase activity that unwinds DNA, allowing access to the repair machinery. Nuclear presence of TFIIH p62 is vital for coordinating transcription and DNA repair processes, highlighting TFIIH p62′s importance in cellular responses to DNA damage and gene expression regulation. Using TFIIH p62 (H-10) monoclonal antibody can provide insights into transcription regulation and DNA repair mechanisms, which are fundamental to understanding various cellular processes and disease states.

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TFIIH p62 Antibody (H-10) References:

1. Assignment of the 1H, 15N, 13C resonances of the N-terminal domain of the human TFIIH P62 subunit.  |  Gervais, V., et al. 2001. J Biomol NMR. 19: 281-2. PMID: 11330817
2. Cloning of the 62-kilodalton component of basic transcription factor BTF2.  |  Fischer, L., et al. 1992. Science. 257: 1392-5. PMID: 1529339
3. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.  |  Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
4. Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.  |  Gerard, M., et al. 1991. J Biol Chem. 266: 20940-5. PMID: 1939143
5. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.  |  Weeda, G., et al. 1990. Cell. 62: 777-91. PMID: 2167179
6. ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.  |  Weber, CA., et al. 1990. EMBO J. 9: 1437-47. PMID: 2184031
7. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
8. Real-time and simultaneous monitoring of the phosphorylation and enhanced interaction of p53 and XPC acidic domains with the TFIIH p62 subunit.  |  Okuda, M. and Nishimura, Y. 2015. Oncogenesis. 4: e150. PMID: 26029824
9. Dataset for the NMR structure of the intrinsically disordered acidic region of XPC bound to the PH domain of TFIIH p62.  |  Okuda, M. and Nishimura, Y. 2016. Data Brief. 6: 571-7. PMID: 26909369
10. The Link of mRNA and rRNA Transcription by PUF60/FIR through TFIIH/P62 as a Novel Therapeutic Target for Cancer.  |  Kitamura, K., et al. 2023. Int J Mol Sci. 24: PMID: 38139171
11. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.  |  Humbert, S., et al. 1994. EMBO J. 13: 2393-8. PMID: 8194529
12. Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH.  |  Marinoni, JC., et al. 1997. EMBO J. 16: 1093-102. PMID: 9118947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIH p62 Antibody (H-10) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIH p62 Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIH p62 Antibody (H-10) Specifications

Alternate Product Name: TFIIH p62

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 249-548

Epitope Target: TFIIH p62

Epitope Species: human

Product Reactivity/Detection: TFIIH p62

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for TFIIH p62 Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent