TEF-1 Antibody (B-2): sc-398227

TEF-1 Antibody (B-2) is a mouse monoclonal IgG2a antibody that detects Transcriptional enhancer factor 1 (TEF-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TEF-1 is a crucial nuclear protein belonging to the TEA/ATTS domain family, known for its role in regulating gene expression across various cell types. TEF-1 (B-2) antibody recognizes a protein featuring a highly conserved DNA-binding domain, allowing interaction with specific DNA sequences such as GT-IIC, SphI/II, and M-CAT, which are essential for transcriptional regulation of genes involved in critical cellular processes. Notably, TEF-1 binds to the proximal regulatory element of transforming growth factor-alpha, a member of the epidermal growth factor family frequently overexpressed in various cancers, highlighting potential implications in tumorigenesis. Additionally, TEF-1 undergoes post-translational modifications, including phosphorylation by several protein kinase C isozymes at serine and threonine residues. This phosphorylation reduces TEF-1′s DNA-binding capability, suggesting a regulatory mechanism that may inhibit transcriptional activity in response to cellular signaling pathways. TEF-1′s ability to dimerize is vital for function as a transcriptional regulator, emphasizing importance in maintaining proper gene expression and cellular homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TEF-1 Antibody (B-2) References:

1. Cooperative binding of TEF-1 to repeated GGAATG-related consensus elements with restricted spatial separation and orientation.  |  Jiang, SW., et al. 2000. DNA Cell Biol. 19: 507-14. PMID: 10975468
2. Cloning and characterization of the mouse alpha1C/A-adrenergic receptor gene and analysis of an alpha1C promoter in cardiac myocytes: role of an MCAT element that binds transcriptional enhancer factor-1 (TEF-1).  |  O'Connell, TD., et al. 2001. Mol Pharmacol. 59: 1225-34. PMID: 11306707
3. M-CAT binding factor is related to the SV40 enhancer binding factor, TEF-1.  |  Farrance, IK., et al. 1992. J Biol Chem. 267: 17234-40. PMID: 1324927
4. Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain.  |  Anbanandam, A., et al. 2006. Proc Natl Acad Sci U S A. 103: 17225-30. PMID: 17085591
5. A TATA-less promoter containing binding sites for ubiquitous transcription factors mediates cell type-specific regulation of the gene for transcription enhancer factor-1 (TEF-1).  |  Boam, DS., et al. 1995. J Biol Chem. 270: 19487-94. PMID: 7642633
6. A TEF-1-independent mechanism for activation of the simian virus 40 (SV40) late promoter by mutant SV40 large T antigens.  |  Casaz, P., et al. 1995. J Virol. 69: 3501-9. PMID: 7745697
7. Characterization of a HeLa cell factor which negatively regulates transcriptional activation in vitro by transcriptional enhancer factor-1 (TEF-1).  |  Chaudhary, S., et al. 1995. J Biol Chem. 270: 3631-7. PMID: 7876100
8. A TEF-1 binding motif that interacts with a placental protein is important for the transcriptional activity of the hCS-B enhancer.  |  Jacquemin, P., et al. 1994. DNA Cell Biol. 13: 1037-45. PMID: 7945936
9. TEF-1 transrepression in BeWo cells is mediated through interactions with the TATA-binding protein, TBP.  |  Jiang, SW. and Eberhardt, NL. 1996. J Biol Chem. 271: 9510-8. PMID: 8621623
10. Human chorionic somatomammotropin enhancer function is mediated by cooperative binding of TEF-1 and CSEF-1 to multiple, low-affinity binding sites.  |  Jiang, SW., et al. 1997. Mol Endocrinol. 11: 1223-32. PMID: 9259314

The preferred TEF-1 antibody is TEF-1 Antibody (H-4): sc-376113.