TEF-1 Antibody (H-4): sc-376113

TEF-1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects TEF-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TEF-1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Transcriptional enhancer factor 1 (TEF-1), a member of the TEA/ATTS domain family, is a nuclear protein that plays a crucial role in regulating gene expression across various cell types. Notably, TEF-1 is involved in binding to specific DNA sequences, including GT-IIC, SphI/II, and M-CAT, as well as the proximal regulatory element of transforming growth factor-alpha, which is often overexpressed in numerous cancers. TEF-1′s ability to repress transcription in placental cells highlights its significance in developmental biology. Additionally, TEF-1 undergoes post-translational modifications, particularly phosphorylation by several protein kinase C isozymes, which occurs at serine and threonine residues. This phosphorylation event reduces TEF-1′s DNA-binding capability, suggesting a potential regulatory role in cellular signaling pathways and tumorigenesis. TEF-1′s capacity to dimerize may be essential for its function as a transcriptional regulator, underscoring its importance in gene expression modulation and cellular processes.

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TEF-1 Antibody (H-4) References:

1. Purification and characterization of TEF1, a transcription factor that controls the human transforming growth factor-alpha promoter.  |  Wang, D. and Kudlow, JE. 1999. Biochim Biophys Acta. 1449: 50-62. PMID: 10076050
2. Novel human TEF-1 isoforms exhibit altered DNA binding and functional properties.  |  Jiang, SW., et al. 2000. Biochemistry. 39: 3505-13. PMID: 10727247
3. Cooperative binding of TEF-1 to repeated GGAATG-related consensus elements with restricted spatial separation and orientation.  |  Jiang, SW., et al. 2000. DNA Cell Biol. 19: 507-14. PMID: 10975468
4. DNA binding of TEA/ATTS domain factors is regulated by protein kinase C phosphorylation in human choriocarcinoma cells.  |  Jiang, SW., et al. 2001. J Biol Chem. 276: 23464-70. PMID: 11313339
5. TEF-1 and MEF2 transcription factors interact to regulate muscle-specific promoters.  |  Maeda, T., et al. 2002. Biochem Biophys Res Commun. 294: 791-7. PMID: 12061776
6. TEF-1 transcription factors regulate activity of the mouse mammary tumor virus LTR.  |  Maeda, T., et al. 2002. Biochem Biophys Res Commun. 296: 1279-85. PMID: 12207913
7. The transcriptional co-activator TAZ interacts differentially with transcriptional enhancer factor-1 (TEF-1) family members.  |  Mahoney, WM., et al. 2005. Biochem J. 388: 217-25. PMID: 15628970
8. MCAT elements and the TEF-1 family of transcription factors in muscle development and disease.  |  Yoshida, T. 2008. Arterioscler Thromb Vasc Biol. 28: 8-17. PMID: 17962623
9. Transcriptional enhancer factor 1 (TEF-1/TEAD1) mediates activation of IFITM3 gene by BRGl.  |  Cuddapah, S., et al. 2008. FEBS Lett. 582: 391-7. PMID: 18177740
10. Repression of involucrin gene expression by transcriptional enhancer factor 1 (TEF-1).  |  Takahashi, H., et al. 1995. Arch Dermatol Res. 287: 740-6. PMID: 8554386
11. DTEF-1, a novel member of the transcription enhancer factor-1 (TEF-1) multigene family.  |  Azakie, A., et al. 1996. J Biol Chem. 271: 8260-5. PMID: 8626520
12. The role of transcription enhancer factor-1 (TEF-1) related proteins in the formation of M-CAT binding complexes in muscle and non-muscle tissues.  |  Farrance, IK. and Ordahl, CP. 1996. J Biol Chem. 271: 8266-74. PMID: 8626521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TEF-1 Antibody (H-4) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-TEF-1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TEF-1 Antibody (H-4) Specifications

Alternate Product Name: TEF-1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 133-173

Epitope Target: TEF-1

Epitope Species: human

Product Reactivity/Detection: TEF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for TEF-1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent