TCR V β 8.1-3 Antibody (F23.1): sc-33648

TCR V β 8.1-3 Antibody (F23.1) is a mouse monoclonal IgG2a kappa light chain antibody that detects the TCR V beta 8.1-3 protein of mouse origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). TCR V β 8.1-3 (F23.1) antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC), providing flexibility for different experimental needs. The T cell antigen receptor (TCR) plays a crucial role in the immune response by recognizing foreign antigens and initiating intracellular signaling pathways that lead to T cell activation. The TCR is a heterodimer, typically composed of either alpha and beta or gamma and delta chains, with the majority of T cells (approximately 95%) expressing the alpha/beta heterodimer. This diversity in TCR specificity is essential for the immune system to effectively recognize a wide array of antigens, achieved through the somatic recombination of variable (V), diversity (D), and joining (J) gene segments during T cell development. The TCR beta chain is synthesized through a precise process that involves the joining of D and J segments, followed by the addition of a V segment, culminating in the incorporation of a constant (C) segment. Notably, genetic mutations in the T cell receptor beta locus have been linked to the development of T cell lymphomas, underscoring the importance of the TCR in both immune function and disease.

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TCR V β 8.1-3 Antibody (F23.1) References:

1. TCR v(beta) repertoire restriction and lack of CDR3 conservation implicate TCR-superantigen interactions in promoting the clonal evolution of murine thymic lymphomas.  |  Gomez, G., et al. 2000. Int Immunol. 12: 263-70. PMID: 10700461
2. Expression of an unusual T cell receptor (TCR)-V beta repertoire by Ly-6C+ subpopulations of CD4+ and/or CD8+ thymocytes. Evidence for a developmental relationship between Ly-6C+ thymocytes and CD4-CD8-TCR-alpha beta+ thymocytes.  |  Takahama, Y., et al. 1991. J Immunol. 147: 2883-91. PMID: 1680922
3. Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.  |  Behlke, MA., et al. 1987. J Exp Med. 165: 257-62. PMID: 2432150
4. Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.  |  Staerz, UD., et al. 1985. J Immunol. 134: 3994-4000. PMID: 2580901
5. Methods for the selection and growth of antigen-specific cytolytic T lines and clones bearing a defined T cell receptor beta chain marker.  |  Ito, M., et al. 1987. J Immunol Methods. 103: 229-37. PMID: 2959725
6. CAR-iNKT cells targeting clonal TCRVβ chains as a precise strategy to treat T cell lymphoma.  |  Rowan, AG., et al. 2023. Front Immunol. 14: 1118681. PMID: 36936927
7. Human T-cell receptor (TCR) alpha/beta + CD4-CD8- T cells express oligoclonal TCRs, share junctional motifs across TCR V beta-gene families, and phenotypically resemble memory T cells.  |  Brooks, EG., et al. 1993. Proc Natl Acad Sci U S A. 90: 11787-91. PMID: 7505446
8. A mouse monoclonal antibody specific for the V beta 5.3 chain of the human TcR recognizes a subgroup of the mouse TcR V beta 8.2 chains.  |  Bleux, C., et al. 1995. J Leukoc Biol. 57: 491. PMID: 7884322
9. Dominant V beta 8 gene usage in response to TNP: failure to use other V beta chains following removal of V beta 8+ T cells by monoclonal antibody in vivo.  |  Dieli, F., et al. 1994. Immunology. 82: 99-105. PMID: 8045598
10. T cell vaccination against autoimmune diabetes in nonobese diabetic mice.  |  Formby, B. and Shao, T. 1993. Ann Clin Lab Sci. 23: 137-47. PMID: 8096128
11. Involvement of TCR-V beta 8.3+ cells in the cure of mice bearing a large MOPC-315 tumor by low dose melphalan.  |  Mokyr, MB., et al. 1993. J Immunol. 151: 4838-46. PMID: 8409442
12. Restricted TCR V beta repertoire in the human mixed lymphocyte reaction is determined by the TCR on the responder cell and not by the stimulating antigen on the stimulator cell.  |  Barth, C., et al. 1996. Transplantation. 61: 133-9. PMID: 8560552

The preferred TCR β antibody is TCR β Antibody (G-11): sc-5277.