TCP-1 β Antibody (D-8): sc-374152

TCP-1 β Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TCP-1 beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-TCP-1 beta antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1 beta, also known as t complex polypeptide 1, is a crucial subunit of the chaperonin containing TCP-1 (CCT) complex, which is essential for proper folding of cytosolic proteins, particularly actins and tubulins. Located in the eukaryotic cytosol, CCT complex comprises eight distinct subunits, each encoded by different genes, and maintains cellular function and integrity. TCP-1 beta′s role in protein folding is particularly important, as misfolded proteins can lead to cellular dysfunction and various diseases. TCP-1 beta was initially identified in mice, where studies linked this protein to tail-less and embryonic lethal phenotypes, and homologous sequences appear in several other species, highlighting evolutionary significance. Studies of TCP-1 beta in yeast demonstrate molecular chaperone activity for actin and tubulin, underscoring this protein′s critical role in cellular processes across different organisms.

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TCP-1 β Antibody (D-8) References:

1. Upregulation of cytosolic chaperonin CCT subunits during recovery from chemical stress that causes accumulation of unfolded proteins.  |  Yokota, SI., et al. 2000. Eur J Biochem. 267: 1658-64. PMID: 10712596
2. Cloning of the chaperonin t-complex polypeptide 1 gene from Schistosoma mansoni and studies of its expression levels under heat shock and oxidative stress.  |  Campos, EG. and Hamdan, FF. 2000. Parasitol Res. 86: 253-8. PMID: 10726997
3. Individual subunits of the eukaryotic cytosolic chaperonin mediate interactions with binding sites located on subdomains of beta-actin.  |  Hynes, GM. and Willison, KR. 2000. J Biol Chem. 275: 18985-94. PMID: 10748209
4. Defining the eukaryotic cytosolic chaperonin-binding sites in human tubulins.  |  Ritco-Vonsovici, M. and Willison, KR. 2000. J Mol Biol. 304: 81-98. PMID: 11071812
5. Two members of the TRiC chaperonin complex, CCT2 and TCP1 are essential for survival of breast cancer cells and are linked to driving oncogenes.  |  Guest, ST., et al. 2015. Exp Cell Res. 332: 223-35. PMID: 25704758
6. Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Protect Liver Ischemia/Reperfusion Injury by Reducing CD154 Expression on CD4+ T Cells via CCT2.  |  Zheng, J., et al. 2020. Adv Sci (Weinh). 7: 1903746. PMID: 32999825
7. CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.  |  Ma, X., et al. 2022. Cell. 185: 1325-1345.e22. PMID: 35366418
8. Reduced ADP off-rate by the yeast CCT2 double mutation T394P/R510H which causes Leber congenital amaurosis in humans.  |  Roy, M., et al. 2023. Commun Biol. 6: 888. PMID: 37644231
9. CCT2 prevented β-catenin proteasomal degradation to sustain cancer stem cell traits and promote tumor progression in epithelial ovarian cancer.  |  Chen, J., et al. 2024. Mol Biol Rep. 51: 54. PMID: 38165547
10. Neuroprotective Effects of Chaperonin Containing TCP1 Subunit 2 (CCT2) on Motor Neurons Following Oxidative or Ischemic Stress.  |  Kwon, HJ., et al. 2024. Neurochem Res. 50: 42. PMID: 39614031
11. Cucumber T-complex protein. Molecular cloning, bacterial expression and characterization within a 22-S cytosolic complex in cotyledons and hypocotyls.  |  Ahnert, V., et al. 1996. Eur J Biochem. 235: 114-9. PMID: 8631317
12. A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development.  |  Iijima, M., et al. 1998. Gene. 213: 101-6. PMID: 9630545

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCP-1 β Antibody (D-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-TCP-1 β Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCP-1 β Antibody (D-8) Specifications

Alternate Product Name: TCP-1 β

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 456-535

Epitope Target: TCP-1 β

Epitope Species: human

Product Reactivity/Detection: TCP-1 β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for TCP-1 β Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin