TCP-1 α Antibody (B-3): sc-374088

TCP-1 α Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TCP-1 α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TCP-1 α (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TCP-1 α, also known as t complex polypeptide 1, is a crucial subunit of the chaperonin containing TCP-1 (CCT) complex, which is essential for protein folding in the eukaryotic cytosol. This complex consists of eight distinct subunits, including TCP-1 α, β, γ, δ, ε, ζ, η, and θ, each encoded by separate genes, highlighting the complexity and specificity of protein folding mechanisms. TCP-1 α plays a vital role in the proper folding of cytosolic proteins, particularly actins and tubulins, which are fundamental for maintaining cellular structure and function. TCP-1 α was first identified in mice with tail-less and embryonic lethal phenotypes, demonstrating its importance in critical developmental processes. Homologous sequences of TCP-1 have been identified across various species, indicating evolutionary conservation and significance in cellular biology. TCP-1′s function as a molecular chaperone for actin and tubulin in both mammalian cells and yeast emphasizes its essential role in ensuring cytoskeletal framework integrity, which is crucial for numerous cellular processes, including motility, division, and intracellular transport.

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TCP-1 α Antibody (B-3) References:

1. Increased expression of cytosolic chaperonin CCT in human hepatocellular and colonic carcinoma.  |  Yokota, S., et al. 2001. Cell Stress Chaperones. 6: 345-50. PMID: 11795471
2. The rate-limiting enzyme in phosphatidylcholine synthesis regulates proliferation of the nucleoplasmic reticulum.  |  Lagace, TA. and Ridgway, ND. 2005. Mol Biol Cell. 16: 1120-30. PMID: 15635091
3. Oxysterols inhibit phosphatidylcholine synthesis via ERK docking and phosphorylation of CTP:phosphocholine cytidylyltransferase.  |  Agassandian, M., et al. 2005. J Biol Chem. 280: 21577-87. PMID: 15788406
4. Changes in macrophage morphology in a Gaucher disease model are dependent on CTP:phosphocholine cytidylyltransferase alpha.  |  Kacher, Y., et al. 2007. Blood Cells Mol Dis. 39: 124-9. PMID: 17482853
5. Nuclear export of the rate-limiting enzyme in phosphatidylcholine synthesis is mediated by its membrane binding domain.  |  Gehrig, K., et al. 2009. J Lipid Res. 50: 966-76. PMID: 19098306
6. Calcium-calmodulin kinase I cooperatively regulates nucleocytoplasmic shuttling of CCTα by accessing a nuclear export signal.  |  Agassandian, M., et al. 2012. Mol Biol Cell. 23: 2755-69. PMID: 22621903
7. ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.  |  Arsenault, DJ., et al. 2013. J Biol Chem. 288: 633-43. PMID: 23155050
8. Environmental hyperosmolality regulates phospholipid biosynthesis in the renal epithelial cell line MDCK.  |  Casali, CI., et al. 2013. J Lipid Res. 54: 677-691. PMID: 23269393
9. Alterations of proteins in MDCK cells during acute potassium deficiency.  |  Peerapen, P., et al. 2016. Biochim Biophys Acta. 1864: 683-696. PMID: 26976750
10. Disease-linked mutations in the phosphatidylcholine regulatory enzyme CCTα impair enzymatic activity and fold stability.  |  Cornell, RB., et al. 2019. J Biol Chem. 294: 1490-1501. PMID: 30559292

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TCP-1 α Antibody (B-3) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TCP-1 α Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TCP-1 α Antibody (B-3) Specifications

Alternate Product Name: TCP-1 α

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 416-525

Epitope Target: TCP-1 α

Epitope Species: human

Product Reactivity/Detection: TCP-1 α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TCP-1 α Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TCP-1 α antibody is TCP-1 α Antibody (91A): sc-53454.