TC-PTP Antibody (F-8): sc-373835
- TC-PTP Antibody (F-8) is a mouse monoclonal IgG2a κ TC-PTP antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 292-415 mapping at the C-terminus of TC-PTP of human origin
- TC-PTP Antibody (F-8) is recommended for detection of TC-PTP of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-TC-PTP Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TC-PTP Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with TC-PTP Antibody (F-8) (see ordering information below).
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TC-PTP Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects TC-PTP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TC-PTP (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. T-cell protein tyrosine phosphatase (TC-PTP) is a crucial non-transmembrane enzyme involved in regulating cellular signaling pathways. TC-PTP exists in two main isoforms, p45TC and p48TC, which are generated through alternative splicing. p45TC isoform is particularly significant as p45TC localizes to the nucleus and plays a vital role in modulating epidermal growth factor receptor (EGFR) signaling pathway activity. Upon EGFR activation, p45TC exits the nucleus and interacts with key substrates, including p52Shc, effectively inhibiting EGF-dependent activation of phosphoinositide 3-kinase and protein kinase B/Akt, which are critical for cell survival and proliferation. In glioblastoma cells, p45TC has been shown to suppress extracellular signal-regulated kinase 2 activation and reduce tumorigenicity in vivo, highlighting p45TC′s potential as a therapeutic target. Additionally, TC-PTP is essential for lymphocyte signaling and maintaining hematopoietic homeostasis, as evidenced by severe hematopoietic defects observed in TC-PTP-deficient mice. TC-PTP gene is located on chromosome 18, underscoring TC-PTP′s importance in various cellular processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TC-PTP Antibody (F-8) References:
- The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. | Tiganis, T., et al. 1999. J Biol Chem. 274: 27768-75. PMID: 10488121
- The protein tyrosine phosphatase TCPTP suppresses the tumorigenicity of glioblastoma cells expressing a mutant epidermal growth factor receptor. | Klingler-Hoffmann, M., et al. 2001. J Biol Chem. 276: 46313-8. PMID: 11514572
- The T cell protein tyrosine phosphatase is a negative regulator of janus family kinases 1 and 3. | Simoncic, PD., et al. 2002. Curr Biol. 12: 446-53. PMID: 11909529
- Cloning and characterization of a mouse cDNA encoding a cytoplasmic protein-tyrosine-phosphatase. | Mosinger, B., et al. 1992. Proc Natl Acad Sci U S A. 89: 499-503. PMID: 1731319
- COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus. | Lorenzen, JA., et al. 1995. J Cell Biol. 131: 631-43. PMID: 7593185
- Isolation and mapping of human T-cell protein tyrosine phosphatase sequences: localization of genes and pseudogenes discriminated using fluorescence hybridization with genomic versus cDNA probes. | Johnson, CV., et al. 1993. Genomics. 16: 619-29. PMID: 8325634
- Epidermal growth factor receptor and the adaptor protein p52Shc are specific substrates of T-cell protein tyrosine phosphatase. | Tiganis, T., et al. 1998. Mol Cell Biol. 18: 1622-34. PMID: 9488479
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TC-PTP Antibody (F-8) | sc-373835 | 200 µg/ml | $316.00 | |||
TC-PTP Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-529583 | 200 µg Ab; 10 µg BP | $354.00 | |||
TC-PTP Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-522522 | 200 µg Ab, 40 µg BP | $354.00 | |||
TC-PTP Antibody (F-8) AC | sc-373835 AC | 500 µg/ml, 25% agarose | $416.00 | |||
TC-PTP Antibody (F-8) HRP | sc-373835 HRP | 200 µg/ml | $316.00 | |||
TC-PTP Antibody (F-8) FITC | sc-373835 FITC | 200 µg/ml | $330.00 | |||
TC-PTP Antibody (F-8) PE | sc-373835 PE | 200 µg/ml | $343.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 488 | sc-373835 AF488 | 200 µg/ml | $357.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 546 | sc-373835 AF546 | 200 µg/ml | $357.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 594 | sc-373835 AF594 | 200 µg/ml | $357.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 647 | sc-373835 AF647 | 200 µg/ml | $357.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 680 | sc-373835 AF680 | 200 µg/ml | $357.00 | |||
TC-PTP Antibody (F-8) Alexa Fluor® 790 | sc-373835 AF790 | 200 µg/ml | $357.00 |