TBS Blotto B: sc-2335
- TBS Blotto B, specifically recommended for use with phospho-specific antibodies, is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mL
- Blocking buffer and antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background in Western blotting applications; preferred over BSA
- Immunoanalytical Grade Blotto contains 1% milk, 1% blocking reagent, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid form
- makes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2O
- To inhibit serine/threonine phosphatase activity, add 50 mM Sodium Flouride (NaF) (sc-24988) to Blotto. And/or Sodium Orthovanadate (Na3O4V) (sc-3540) to inhibit protein tyrosine phosphatase activity. Alternatively, add Phosphatase Inhibitor Cocktail A (sc-45044) and Phosphatase Inhibitor Cocktail B (sc-45045)
- Recommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using HRP conjugated primary and secondary antibodies, or mouse IgG binding proteins
- If using direct conjugated primary antibodies or for near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent (sc-516214)
- If not blotting with phospho-specific antibodies, we recommend our alternative product, TBS Blotto A (sc-2333) which contains a unique formulation of 5% milk, 0.05% Tween-20
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TBS Blotto B is a blocking solution commonly used in Western blotting and other immunoassay techniques, similar to TBS Blotto A. It typically contains Tris-buffered saline (TBS), which consists of Tris-HCl and NaCl, along with a blocking agent such as non-fat dry milk or bovine serum albumin (BSA). In Western blotting, TBS Blotto B serves the same purpose as TBS Blotto A: to block non-specific binding sites on the membrane, thereby reducing background noise and increasing the specificity and sensitivity of antibody detection. TBS Blotto B is added to the membrane after the primary antibody incubation step and before the secondary antibody incubation step in Western blotting protocols. It is incubated with the membrane for a period of time to ensure complete blocking of non-specific binding sites. Similarly to TBS Blotto A, TBS Blotto B can also be used as a diluent for primary and secondary antibodies, as well as for washing steps in immunoassay procedures. Its composition provides a suitable environment for antibody-antigen interactions and helps maintain the stability of proteins during the assay.
TBS Blotto B References:
- Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. | Jeong, HJ., et al. 2002. J Agric Food Chem. 50: 5903-8. PMID: 12358457
- Characterization of lunasin isolated from soybean. | Jeong, HJ., et al. 2003. J Agric Food Chem. 51: 7901-6. PMID: 14690371
- The cancer preventive peptide lunasin from wheat inhibits core histone acetylation. | Jeong, HJ., et al. 2007. Cancer Lett. 255: 42-8. PMID: 17481808
- Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb). | Jeong, JB., et al. 2007. J Agric Food Chem. 55: 10707-13. PMID: 18038993
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBS Blotto B | sc-2335 | makes 500 ml | $26.00 |