Date published: 2025-10-20

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Phosphatase Inhibitor Cocktail B

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Alternate Names:
Phosphatase Inhibitor Cocktail B also known as Phosphatase Inhibitor Cocktail II; Phosphatase Inhibitor Cocktail Set II
Application:
Phosphatase Inhibitor Cocktail B is an inhibitor of PTP-ases as well as acid and alkaline phosphatases
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Phosphatase Inhibitor Cocktail B is a cocktail of five phosphatase inhibitors which has been optimized for the inhibition of protein tyrosine phosphatases, as well as acid and alkaline phosphatases. It can be used on animal tissues, A431, or Jurkat cell extracts. Phosphatase Inhibitor Cocktail B is suitable for cell extracts containing detergents. Each vial (reconstituted in 1ml) contains 200 mM Imidazole, 100 mM Sodium Fluoride, 115 mM Sodium Molybdate, 100 mM Sodium Orthovanadate and 400 mM Sodium Tartrate Dihydrate. Because Phosphatase Inhibitor Cocktail B is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


Phosphatase Inhibitor Cocktail B References

  1. The absolute quantification strategy: application to phosphorylation profiling of human separase serine 1126.  |  Gerber, SA., et al. 2007. Methods Mol Biol. 359: 71-86. PMID: 17484111
  2. Elucidation of the mechanism of ribose conjugation in a pyrazole-containing compound in rodent liver.  |  Le, H., et al. 2013. Xenobiotica. 43: 236-45. PMID: 22931212
  3. Sunitinib Treatment Exacerbates Intratumoral Heterogeneity in Metastatic Renal Cancer.  |  Stewart, GD., et al. 2015. Clin Cancer Res. 21: 4212-23. PMID: 26015515
  4. Class II phosphoinositide 3-kinase C2β regulates a novel signaling pathway involved in breast cancer progression.  |  Chikh, A., et al. 2016. Oncotarget. 7: 18325-45. PMID: 26934321
  5. Novel roles for class II Phosphoinositide 3-Kinase C2β in signalling pathways involved in prostate cancer cell invasion.  |  Mavrommati, I., et al. 2016. Sci Rep. 6: 23277. PMID: 26983806
  6. Oxyresveratrol Supplementation to C57bl/6 Mice Fed with a High-Fat Diet Ameliorates Obesity-Associated Symptoms.  |  Tan, HY., et al. 2017. Nutrients. 9: PMID: 28212343
  7. Thiamine Deprivation Produces a Liver ATP Deficit and Metabolic and Genomic Effects in Mice: Findings Are Parallel to Those of Biotin Deficiency and Have Implications for Energy Disorders.  |  Hernandez-Vazquez, AJ., et al. 2016. J Nutrigenet Nutrigenomics. 9: 287-299. PMID: 28214879
  8. Chitosan based micelle with zeta potential changing property for effective mucosal drug delivery.  |  Le-Vinh, B., et al. 2019. Int J Biol Macromol. 133: 647-655. PMID: 30986465
  9. linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.  |  Chen, X., et al. 2021. Cell Rep. 34: 108584. PMID: 33406422
  10. Follicle-stimulating hormone worsens osteoarthritis by causing inflammation and chondrocyte dedifferentiation.  |  Huan, Z., et al. 2021. FEBS Open Bio. 11: 2292-303. PMID: 34176242
  11. Transgenic Mice Expressing Human α-Synuclein 1-103 Fragment as a Novel Model of Parkinson's Disease.  |  Tian, Y., et al. 2021. Front Aging Neurosci. 13: 760781. PMID: 34744697
  12. SARS-CoV-2 spike protein harnesses SNX27-mediated endocytic recycling pathway.  |  Zhao, L., et al. 2021. MedComm (2020). 2: 798-809. PMID: 34909756
  13. 7,8-Dihydroxyflavone ameliorates mitochondrial impairment and motor dysfunction in the α-synuclein 1-103 transgenic mice.  |  Tian, Y., et al. 2022. Neurobiol Dis. 169: 105736. PMID: 35462005

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Phosphatase Inhibitor Cocktail B, 1 vial

sc-45045
1 vial
$69.00

Can this be added to a cell suspension of live cells or in whole blood? Will it cause cell aggregation, clotting and/or cell lysis?

Asked by: verycurious
Thank you for your question. This antibody has only been analyzed for its properties in detection assays. We do not have any data on its biological functions with live cells. For cell culture work with any of our antibody products, please note that they are supplied in a buffer containing 0.1 % sodium azide, which may be detrimental to certain cell types.
Answered by: Tech Support Europe
Date published: 2023-01-19

I just ordered this product and i want to know that after Reconstitute each vial with 1 ml H2O to obtain a 1 ml stock solution.How can i storage at -20 or 4oC? and i want to add in RIPA buffer for extraction of protein so i want a procedure to use it .

Asked by: serene
Thank you for your question. Following reconstitution, aliquot and freeze (-20ºC). Stock solutions are stable for up to 1 month at -20ºC. Please refer to the tab "Technical Information" for usage instructions shown under our own RIPA Lysis Buffer System on our website. See link below. https://www.scbt.com/p/ripa-lysis-buffer-system
Answered by: Technical Support
Date published: 2021-01-29
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Rated 3 out of 5 by from Works well for WBVery economical to use with clean WB results. Lasts for ever as very minimal quantity is needed in the lysis buffer
Date published: 2019-03-14
Rated 5 out of 5 by from Great productI bought that items and so happy with the performance and results
Date published: 2017-03-07
Rated 5 out of 5 by from I use the Phosphatase Inhibitor CocktailI use the Phosphatase Inhibitor Cocktail B together with Phosphatase Inhibitor Cocktail A (45044) on my cell lysates. The inhibition works very well! I can clearly detect phosphorylation on my Western Blots
Date published: 2015-10-13
Rated 5 out of 5 by from By having this Phosphatase Inhibitor CocktailBy having this Phosphatase Inhibitor Cocktail B present in the cell lysate, de-phosphorylation is prevented by any phosphatase enzymes that may be present in the sample. This action is demonstrated by treating K-562 cell lysate (containing this inhibitor as part of the lysis buffer) with lambda protein phosphatase to see if the phosphorylation of the Cofilin 1 protein can be altered. By WB, we can see that this inhibitor cocktail does indeed prevent lambda protein phosphatase from de-phosphorylating p-Cofilin 1 protein. -SCBT QC
Date published: 2015-01-27
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Phosphatase Inhibitor Cocktail B is rated 4.5 out of 5 by 4.
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