Date published: 2025-10-20

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Phosphatase Inhibitor Cocktail A

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Alternate Names:
Phosphatase Inhibitor Cocktail I
Application:
Phosphatase Inhibitor Cocktail A is an inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.Each vial contains 2.5 mM (−)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


Phosphatase Inhibitor Cocktail A References

  1. Cucurbitacin I inhibits Stat3 and induces apoptosis in Sézary cells.  |  van Kester, MS., et al. 2008. J Invest Dermatol. 128: 1691-5. PMID: 18200050
  2. Antitumor effects of Mucin 1/sec involves the modulation of urokinase-type plasminogen activator and signal transducer and activator of transcription 1 expression in tumor cells.  |  Ilkovitch, D., et al. 2008. Cancer Res. 68: 2427-35. PMID: 18381451
  3. Rapid purification of protein phosphatase 2A from mouse brain by microcystin-affinity chromatography.  |  Nishiwaki, S., et al. 1991. FEBS Lett. 279: 115-8. PMID: 1847341
  4. Role of c-Abl kinase in DNA mismatch repair-dependent G2 cell cycle checkpoint arrest responses.  |  Wagner, MW., et al. 2008. J Biol Chem. 283: 21382-93. PMID: 18480061
  5. S-trityl-L-cysteine derivative induces caspase-independent cell death in K562 human chronic myeloid leukemia cell line.  |  Shimizu, M., et al. 2010. Cancer Lett. 298: 99-106. PMID: 20619960
  6. Non-histone lysine acetylated proteins in heart failure.  |  Grillon, JM., et al. 2012. Biochim Biophys Acta. 1822: 607-14. PMID: 22155497
  7. TLR4 signaling and the inhibition of liver hepcidin expression by alcohol.  |  Zmijewski, E., et al. 2014. World J Gastroenterol. 20: 12161-70. PMID: 25232250
  8. Class II phosphoinositide 3-kinase C2β regulates a novel signaling pathway involved in breast cancer progression.  |  Chikh, A., et al. 2016. Oncotarget. 7: 18325-45. PMID: 26934321
  9. A targeted proteomics approach to the quantitative analysis of ERK/Bcl-2-mediated anti-apoptosis and multi-drug resistance in breast cancer.  |  Yang, T., et al. 2016. Anal Bioanal Chem. 408: 7491-503. PMID: 27510278
  10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE.  |  Kosako, H. and Motani, K. 2017. Methods Mol Biol. 1487: 137-149. PMID: 27924564
  11. Surface charge tuneable fluorescent protein-based logic gates for smart delivery of nucleic acids.  |  Zhao, K., et al. 2017. Chem Commun (Camb). 53: 11326-11329. PMID: 28967021
  12. Factors other than hTau overexpression that contribute to tauopathy-like phenotype in rTg4510 mice.  |  Gamache, J., et al. 2019. Nat Commun. 10: 2479. PMID: 31171783
  13. Analysis of cardiac troponin proteoforms by top-down mass spectrometry.  |  Tiambeng, TN., et al. 2019. Methods Enzymol. 626: 347-374. PMID: 31606082
  14. Nanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum.  |  Tiambeng, TN., et al. 2020. Nat Commun. 11: 3903. PMID: 32764543
  15. SARS-CoV-2 spike protein harnesses SNX27-mediated endocytic recycling pathway.  |  Zhao, L., et al. 2021. MedComm (2020). 2: 798-809. PMID: 34909756
  16. Handbook of Enzyme Inhibitors  |  Mahendra Kumar Jain. 1982. John Wiley & Sons. 1: 222, 334.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Phosphatase Inhibitor Cocktail A, 1 ml

sc-45044
1 ml
$105.00

Can I use phosphatase inhibitor A in RIPA buffer with phosphatase inhibitor B?

Asked by: Chorrillo
Thank you for Yes, this would work and if you need further assistance please contact technical service by emailing scbt@scbt.com or (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-04-12

how should I store this inhibitor (-20 I know)--- Do I need to aliquot ?is it sensitive to  freeze thaw? In Blotto B your protocol calls for a dil. of 0.01%(v/v) yet other places I see dilute 100 fold. How long is it stable at 4 degrees in Blotto B?

Asked by: Lyndle
For information on chemical storage please contact Technical Service
Answered by: Tech Sales
Date published: 2021-05-12

Can this product ship to China?

Asked by: SCchem16
Thank you for your question. The compound, sc-45044, cannot ship overseas due to the presence of Microcystin-LR. A possible alternative which can be shipped overseas is sc-45065. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-24
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Rated 4 out of 5 by from Works wellFollowed the Instructions and it works well for making lysates WB
Date published: 2021-02-18
Rated 5 out of 5 by from Great phosphatase inhibitorGreat phosphatase inhibitor! I add it on cell extracts 1:100 dilution, as its recommended, and works well!
Date published: 2015-10-13
Rated 5 out of 5 by from Used with frozen tissue sections and worked wellUsed with frozen tissue sections and worked well.
Date published: 2015-06-03
Rated 5 out of 5 by from By having this Phosphatase Inhibitor CocktailBy having this Phosphatase Inhibitor Cocktail A present in the cell lysate, de-phosphorylation is prevented by any phosphatase enzymes that may be present in the sample. This action is demonstrated by treating K-562 cell lysate (containing this inhibitor as part of the lysis buffer) with lambda protein phosphatase to see if the phosphorylation of the Cofilin 1 protein can be altered. By WB, we can see that this inhibitor cocktail does indeed prevent lambda protein phosphatase from de-phosphorylating p-Cofilin 1 protein. -SCBT QC
Date published: 2015-03-01
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Phosphatase Inhibitor Cocktail A is rated 4.8 out of 5 by 4.
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