TBC1D8 Antibody (B-5): sc-376637

TBC1D8 Antibody (B-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects TBC1D8 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TBC1D8 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TBC1D8, also known as VRP (Vascular Rab-GAP/TBC-containing protein), AD3, or HBLP1, is a crucial GTPase-activating protein that plays a significant role in regulating the activity of Rab proteins, which are essential for intracellular vesicle trafficking. By accelerating the intrinsic rate of GTP hydrolysis, TBC1D8 downregulates the active form of these Rab proteins, thereby influencing various cellular processes such as membrane transport and signal transduction. TBC1D8 consists of 897 amino acids and features a highly conserved Rab-GAP TBC domain, which is vital for catalytic activity. Additionally, TBC1D8 contains a GRAM domain that may be involved in membrane association, further underscoring importance in cellular dynamics. The gene encoding TBC1D8 is located on human chromosome 2, a region that encompasses over 1,400 genes and represents nearly 8% of the human genome, highlighting potential involvement in a wide array of biological functions and relevance in health and disease.

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TBC1D8 Antibody (B-5) References:

1. Antisense display--a method for functional gene screening: evaluation in a cell-free system and isolation of angiogenesis-related genes.  |  Yonekura, H., et al. 1999. Nucleic Acids Res. 27: 2591-600. PMID: 10373574
2. Identification of the catalytic domains and their functionally critical arginine residues of two yeast GTPase-activating proteins specific for Ypt/Rab transport GTPases.  |  Albert, S., et al. 1999. EMBO J. 18: 5216-25. PMID: 10508155
3. Involvement of TRAF4 in oxidative activation of c-Jun N-terminal kinase.  |  Xu, YC., et al. 2002. J Biol Chem. 277: 28051-7. PMID: 12023963
4. Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity.  |  Itoh, T., et al. 2006. Genes Cells. 11: 1023-37. PMID: 16923123
5. A Rab-GAP TBC domain protein binds hepatitis C virus NS5A and mediates viral replication.  |  Sklan, EH., et al. 2007. J Virol. 81: 11096-105. PMID: 17686842
6. Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity.  |  Ishibashi, K., et al. 2009. Genes Cells. 14: 41-52. PMID: 19077034

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TBC1D8 Antibody (B-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TBC1D8 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TBC1D8 Antibody (B-5) Specifications

Alternate Product Name: TBC1D8

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 701-763

Epitope Target: TBC1D8

Epitope Species: human

Product Reactivity/Detection: TBC1D8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TBC1D8 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin