Tau Antibody (D-8): sc-166060

Tau Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody designed to recognize Tau protein, also known as MAPT (microtubule-associated protein tau), or TAU. Tau monoclonal antibody (D-8) targets the amino acids 1-150 region of the human Tau protein and is effective in detecting Tau from mouse, rat, and human sources using techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, anti-Tau antibody (D-8) offers versatile applications for diverse experimental needs. Tau is a 758 amino acid protein predominantly expressed in neuronal tissues, where Tau plays a crucial role in promoting microtubule assembly and stability, maintaining neuronal polarity, and facilitating cytoskeletal plasticity by linking microtubules to plasma membrane components. Tau protein exists in multiple alternatively spliced isoforms and is localized to the cytoplasm, cytoskeleton, and cell membrane. Tau undergoes several post-translational modifications, such as phosphorylation on serine and threonine residues, polyubiquitination leading to proteasomal degradation, and glycation of specific isoforms. These modifications are essential for regulating Tau′s binding to microtubules, degradation, and ability to form aggregates, which are critical factors in normal function and involvement in disease processes. Dysregulation of these post-translational modifications is associated with neurodegenerative disorders, including Alzheimer′s disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD), and progressive supranuclear palsy (PSP). Tau monoclonal antibody (D-8) is an invaluable tool for researchers studying the physiological roles of Tau and contribution to various neurological diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tau Antibody (D-8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Tau Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tau Antibody (D-8) Specifications

Alternate Product Name: MAPT

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-150

Epitope Target: Tau

Epitope Species: human

Product Reactivity/Detection: Tau

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Tau Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Tau antibody is Tau Antibody (Tau 46): sc-32274.