TARDBP Antibody (E-10): sc-376311

TARDBP Antibody (E-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects TARDBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-TARDBP antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TARDBP, also known as TDP-43, is a crucial nuclear protein characterized by two RNA recognition motif (RRM) domains, which play a significant role in RNA processing and regulation. TARDBP is ubiquitously expressed, with highest levels found in placenta, lung, pancreas, spleen, and genital tract, and is essential for binding to TAR DNA sequence motifs of HIV, functioning as a transcriptional repressor that inhibits HIV-1 transcription. TARDBP is involved in splicing of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, highlighting TARDBP′s importance in maintaining proper cellular function. TARDBP is a major component of ubiquitin-positive inclusion bodies, which are associated with various neurodegenerative diseases, suggesting TARDBP′s potential role in disease pathogenesis. Due to alternative splicing, multiple isoforms of TARDBP exist, further emphasizing TARDBP′s complexity and significance in cellular processes and disease mechanisms.

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TARDBP Antibody (E-10) References:

1. Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping.  |  Buratti, E., et al. 2001. EMBO J. 20: 1774-84. PMID: 11285240
2. Structural diversity and functional implications of the eukaryotic TDP gene family.  |  Wang, HY., et al. 2004. Genomics. 83: 130-9. PMID: 14667816
3. Variation in a repeat sequence determines whether a common variant of the cystic fibrosis transmembrane conductance regulator gene is pathogenic or benign.  |  Groman, JD., et al. 2004. Am J Hum Genet. 74: 176-9. PMID: 14685937
4. Nuclear factor TDP-43 binds to the polymorphic TG repeats in CFTR intron 8 and causes skipping of exon 9: a functional link with disease penetrance.  |  Buratti, E., et al. 2004. Am J Hum Genet. 74: 1322-5. PMID: 15195661
5. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis.  |  Neumann, M., et al. 2006. Science. 314: 130-3. PMID: 17023659
6. Pathological TDP-43 distinguishes sporadic amyotrophic lateral sclerosis from amyotrophic lateral sclerosis with SOD1 mutations.  |  Mackenzie, IR., et al. 2007. Ann Neurol. 61: 427-34. PMID: 17469116
7. TARDBP mutations in individuals with sporadic and familial amyotrophic lateral sclerosis.  |  Kabashi, E., et al. 2008. Nat Genet. 40: 572-4. PMID: 18372902
8. TARDBP (TDP-43) sequence analysis in patients with familial and sporadic ALS: identification of two novel mutations.  |  Del Bo, R., et al. 2009. Eur J Neurol. 16: 727-32. PMID: 19236453
9. FUS and TARDBP but not SOD1 interact in genetic models of amyotrophic lateral sclerosis.  |  Kabashi, E., et al. 2011. PLoS Genet. 7: e1002214. PMID: 21829392
10. Loss-of-function mutation in VCP mimics the characteristic pathology as in FTLD-TARDBP.  |  Wani, A. and Weihl, CC. 2021. Autophagy. 17: 4502-4503. PMID: 34632910
11. TARDBP is a candidate diagnostic biomarker promoting tumor progression via impacting tumor immunity and tumor microenvironment.  |  Luo, MY., et al. 2024. J Cancer. 15: 4113-4127. PMID: 38947395
12. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs.  |  Ou, SH., et al. 1995. J Virol. 69: 3584-96. PMID: 7745706

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TARDBP Antibody (E-10) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-TARDBP Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TARDBP Antibody (E-10) Specifications

Alternate Product Name: TDP-43

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-89

Epitope Target: TARDBP

Epitope Species: human

Product Reactivity/Detection: TARDBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for TARDBP Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred TARDBP antibody is TARDBP Antibody (41-7.1): sc-100871.