TAP Antibody (53H8): sc-32319

TAP Antibody (53H8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-TAP antibody (53H8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TAP protein, also known as NXF1, plays a crucial role in the export of mRNA from the nucleus to the cytoplasm, which is essential for proper gene expression and cellular function. TAP interacts with the constitutive transport element (CTE) of type D retroviruses, facilitating the nuclear export of unspliced viral RNAs. This interaction enables efficient viral replication and production of new virions. TAP belongs to a larger family of NXF proteins, characterized by conserved leucine-rich-repeat domain and nuclear transport factor 2-like domain, highlighting the evolutionary significance of this protein family in cellular transport mechanisms.

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TAP Antibody (53H8) References:

1. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.  |  Kang, Y. and Cullen, BR. 1999. Genes Dev. 13: 1126-39. PMID: 10323864
2. Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor.  |  Kang, Y., et al. 1999. Virology. 262: 200-9. PMID: 10489353
3. Regulation of Ped gene expression by TAP protein.  |  Ke, X. and Warner, CM. 2000. J Reprod Immunol. 46: 1-15. PMID: 10708239
4. TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.  |  Herold, A., et al. 2000. Mol Cell Biol. 20: 8996-9008. PMID: 11073998
5. The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.  |  Tan, W., et al. 2000. RNA. 6: 1762-72. PMID: 11142376
6. Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.  |  Braun, IC., et al. 2001. J Biol Chem. 276: 20536-43. PMID: 11259411
7. Identification of a novel posttranscriptional regulatory element by using a rev- and RRE-mutated human immunodeficiency virus type 1 DNA proviral clone as a molecular trap.  |  Nappi, F., et al. 2001. J Virol. 75: 4558-69. PMID: 11312326
8. Retroviral constitutive transport element evolved from cellular TAP(NXF1)-binding sequences.  |  Zolotukhin, AS., et al. 2001. J Virol. 75: 5567-75. PMID: 11356964
9. ICP47 mediates viral neuroinvasiveness by induction of TAP protein following intravenous inoculation of herpes simplex virus type 1 in mice.  |  Burgos, JS., et al. 2006. J Neurovirol. 12: 420-7. PMID: 17162658
10. The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable.  |  Johnson, LA., et al. 2009. J Virol. 83: 6335-46. PMID: 19369354
11. Analyses of conformational states of the transporter associated with antigen processing (TAP) protein in a native cellular membrane environment.  |  Geng, J., et al. 2013. J Biol Chem. 288: 37039-47. PMID: 24196954
12. Structure and function of the TAP protein and related Ly-6-linked molecules.  |  Reiser, H., et al. 1988. Year Immunol. 3: 80-8. PMID: 2831682

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAP Antibody (53H8) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-TAP Antibody (53H8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAP Antibody (53H8) Specifications

Alternate Product Name: TAP

Product Clone Name: 53H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TAP

Epitope Species: human

Product Reactivity/Detection: TAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for TAP Antibody (53H8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin