TANK Antibody (H-10): sc-374189

TANK Antibody (H-10) is a mouse monoclonal IgG1 antibody that detects TANK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TANK, or TRAF family member-associated NFKB activator, plays a crucial role in the regulation of the NFkB signaling pathway, which is vital for immune response, inflammation, and cell survival. Located primarily in the cytoplasm, TANK interacts with TRAF proteins, particularly TRAF2, to facilitate the activation of NFkB upon stimulation by ligands such as TNF or CD40L. TANK′s cellular location enables functioning as a regulatory hub, remaining inactive in unstimulated cells due to its carboxy-terminal domain. Upon binding to TRAF2, TANK undergoes a conformational change that releases this inhibition, allowing for the subsequent activation of NFkB. This mechanism highlights TANK′s essential function in modulating immune signaling and underscores TANK′s potential as a target for therapeutic interventions in diseases characterized by dysregulated NFkB activity. Anti-TANK antibody (H-10) serves as an invaluable tool for researchers investigating the complexities of immune signaling pathways and their implications in various biological processes.

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TANK Antibody (H-10) References:

1. NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase.  |  Pomerantz, JL. and Baltimore, D. 1999. EMBO J. 18: 6694-704. PMID: 10581243
2. NF-kappaB activation through IKK-i-dependent I-TRAF/TANK phosphorylation.  |  Nomura, F., et al. 2000. Genes Cells. 5: 191-202. PMID: 10759890
3. Constitutive and interleukin-1-inducible phosphorylation of p65 NF-{kappa}B at serine 536 is mediated by multiple protein kinases including I{kappa}B kinase (IKK)-{alpha}, IKK{beta}, IKK{epsilon}, TRAF family member-associated (TANK)-binding kinase 1 (TBK1), and an unknown kinase and couples p65 to TATA-binding protein-associated factor II31-mediated interleukin-8 transcription.  |  Buss, H., et al. 2004. J Biol Chem. 279: 55633-43. PMID: 15489227
4. The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1.  |  Zhang, W., et al. 2010. Mol Biol Cell. 21: 2500-13. PMID: 20484576
5. Deregulated expression of TANK in glioblastomas triggers pro-tumorigenic ERK1/2 and AKT signaling pathways.  |  Stellzig, J., et al. 2013. Oncogenesis. 2: e79. PMID: 24217713
6. TRAF Family Member-associated NF-κB Activator (TANK) Inhibits Genotoxic Nuclear Factor κB Activation by Facilitating Deubiquitinase USP10-dependent Deubiquitination of TRAF6 Ligase.  |  Wang, W., et al. 2015. J Biol Chem. 290: 13372-85. PMID: 25861989
7. Encephalomyocarditis Virus 3C Protease Relieves TRAF Family Member-associated NF-κB Activator (TANK) Inhibitory Effect on TRAF6-mediated NF-κB Signaling through Cleavage of TANK.  |  Huang, L., et al. 2015. J Biol Chem. 290: 27618-32. PMID: 26363073
8. The Cortical Neuroimmune Regulator TANK Affects Emotional Processing and Enhances Alcohol Drinking: A Translational Study.  |  Müller, CP., et al. 2019. Cereb Cortex. 29: 1736-1751. PMID: 30721969
9. TANK Promotes Pressure Overload Induced Cardiac Hypertrophy via Activating AKT Signaling Pathway.  |  Pang, Y., et al. 2021. Front Cardiovasc Med. 8: 687540. PMID: 34540911
10. TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation.  |  Cheng, G. and Baltimore, D. 1996. Genes Dev. 10: 963-73. PMID: 8608943

The preferred TANK antibody is TANK Antibody (D-2): sc-166643.