TANK Antibody (D-2): sc-166643

TANK Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects TANK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TANK (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TANK protein plays a crucial role in the regulation of the NFκB signaling pathway, which is vital for immune response, inflammation, and cell survival. Located primarily in the cytoplasm, TANK acts as a scaffold protein that interacts with TRAF family members, particularly TRAF2, to facilitate NFκB activation in response to tumor necrosis factor (TNF) receptor stimulation. This interaction is essential for the transduction of signals that lead to the transcription of genes involved in immune and inflammatory responses. Notably, TANK contains a regulatory carboxy-terminal domain that keeps TANK inactive in unstimulated cells, and TANK′s association with TRAF2 alleviates this inhibition, allowing for the activation of downstream signaling pathways. Understanding TANK′s function and interactions with TRAF proteins is significant for elucidating the mechanisms of immune regulation and could provide insights into therapeutic targets for inflammatory diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TANK Antibody (D-2) References:

1. The TFG protein, involved in oncogenic rearrangements, interacts with TANK and NEMO, two proteins involved in the NF-kappaB pathway.  |  Miranda, C., et al. 2006. J Cell Physiol. 208: 154-60. PMID: 16547966
2. SRY-box containing gene 11 (Sox11) transcription factor is required for neuron survival and neurite growth.  |  Jankowski, MP., et al. 2006. Neuroscience. 143: 501-14. PMID: 17055661
3. TRAF Family Member-associated NF-κB Activator (TANK) Inhibits Genotoxic Nuclear Factor κB Activation by Facilitating Deubiquitinase USP10-dependent Deubiquitination of TRAF6 Ligase.  |  Wang, W., et al. 2015. J Biol Chem. 290: 13372-85. PMID: 25861989
4. Encephalomyocarditis Virus 3C Protease Relieves TRAF Family Member-associated NF-κB Activator (TANK) Inhibitory Effect on TRAF6-mediated NF-κB Signaling through Cleavage of TANK.  |  Huang, L., et al. 2015. J Biol Chem. 290: 27618-32. PMID: 26363073
5. Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1.  |  Fu, T., et al. 2018. Proc Natl Acad Sci U S A. 115: E11651-E11660. PMID: 30459273
6. TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40.  |  Rothe, M., et al. 1995. Science. 269: 1424-7. PMID: 7544915
7. Contenders in FasL/TNF death signaling.  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
8. The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death.  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
9. Transducers of life and death: TNF receptor superfamily and associated proteins.  |  Baker, SJ. and Reddy, EP. 1996. Oncogene. 12: 1-9. PMID: 8552378
10. B cells from p50/NF-kappa B knockout mice have selective defects in proliferation, differentiation, germ-line CH transcription, and Ig class switching.  |  Snapper, CM., et al. 1996. J Immunol. 156: 183-91. PMID: 8598461
11. TANK, a co-inducer with TRAF2 of TNF- and CD 40L-mediated NF-kappaB activation.  |  Cheng, G. and Baltimore, D. 1996. Genes Dev. 10: 963-73. PMID: 8608943
12. Human dendritic cells activate T lymphocytes via a CD40: CD40 ligand-dependent pathway.  |  McLellan, AD., et al. 1996. Eur J Immunol. 26: 1204-10. PMID: 8647193

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TANK Antibody (D-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-TANK Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TANK Antibody (D-2) Specifications

Alternate Product Name: TANK

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 126-425

Epitope Target: TANK

Epitope Species: human

Product Reactivity/Detection: TANK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for TANK Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin