TAF II p250 Antibody (6B3): sc-735

TAF II p250 Antibody (6B3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAF II p250 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). TAF II p250 monoclonal antibody (6B3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. TAF II p250 plays a crucial role in transcriptional regulation of genes, particularly those involved in cell cycle progression, by being a part of the TFIID complex, which is essential for assembly of the pre-initiation complex at the TATA promoter. TAF II p250 structure consists of multiple subunits, including the TATA-binding protein and various TBP-associated factors, which together facilitate both basal and activator-dependent transcription. TAF II p250 has been identified as identical to CCG1, a nuclear DNA-binding protein vital for proper cell cycle regulation. TAF II p250 interaction with specific transcription factors allows for precise activation of genes necessary for cell cycle progression, underscoring importance in cellular function and development.

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TAF II p250 Antibody (6B3) References:

1. Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau).  |  Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
2. Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation.  |  Dynlacht, BD., et al. 1991. Cell. 66: 563-76. PMID: 1907890
3. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein.  |  Sekiguchi, T., et al. 1991. Mol Cell Biol. 11: 3317-25. PMID: 2038334
4. Five intermediate complexes in transcription initiation by RNA polymerase II.  |  Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
5. Molecular cloning of the cDNA of human X chromosomal gene (CCG1) which complements the temperature-sensitive G1 mutants, tsBN462 and ts13, of the BHK cell line.  |  Sekiguchi, T., et al. 1988. EMBO J. 7: 1683-7. PMID: 3169001
6. Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.  |  Matsui, T., et al. 1980. J Biol Chem. 255: 11992-6. PMID: 7440580
7. Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation.  |  Ruppert, S., et al. 1993. Nature. 362: 175-9. PMID: 7680771
8. The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1.  |  Hisatake, K., et al. 1993. Nature. 362: 179-81. PMID: 8450888

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAF II p250 Antibody (6B3) has 69 citations in a variety of scientific publications.

Primary antibodies like Anti-TAF II p250 Antibody (6B3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAF II p250 Antibody (6B3) Specifications

Alternate Product Name: TAF II p250

Product Clone Name: 6B3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TAF II p250

Epitope Species: human

Product Reactivity/Detection: TAF II p250

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 69 citations in various scientific publications

Available Conjugates/Forms for TAF II p250 Antibody (6B3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent