Anti-TAF II p250 Antibody (6B3) is a mouse monoclonal IgG1 κ TAF II p250 antibody, cited in 63 publications, provided at 200 µg/ml
raised against TAF II p250 isolated from HeLa cells
TAF II p250 Antibody (6B3) is recommended for detection of TAF II p250 of human origin by WB, IP, IF and IHC(P); non cross-reactive with TAF II p250 of hamster or Drosophila origin
Anti-TAF II p250 Antibody (6B3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-735 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TAF II p250 (6B3): sc-735.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for TAF II p250 Antibody (6B3) for WB and IHC(P) applications. These reagents are now offered in bundles with TAF II p250 Antibody (6B3) (see ordering information below).
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TAF II p250 Antibody (6B3) is a high quality monoclonal TAF II p250 antibody (also designated TAF II p250 antibody) suitable for the detection of the TAF II p250 protein of human origin. TAF II p250 Antibody (6B3) is available as both the non-conjugated anti-TAF II p250 antibody form, as well as multiple conjugated forms of anti-TAF II p250 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. TFIID is a general transcription factor which initiates pre-initiation complex assembly through direct interaction with the TATA promoter element. It is a multi-subunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. The largest subunit (TAF) of TFIID is a protein designated TAF II p250. Of interest, TAF II p250 has been cloned and shown to be identical to CCG1, a nuclear DNA-binding protein known to be important for cell cycle progression. This part of TAF II p250 may serve a specific function in activation of a subset of genes important for cell cycle progression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can you let me know what was the epitope used to produce this antibody
Asked by: Anonymous
Thank you for your question. TAF II p250 Antibody (6B3): sc-735 was raised against TAF II p250 isolated from HeLa cells. Its epitope region has not been determined yet.
Answered by: Tech Support Europe
Date published: 2019-11-15
Can the TransCruz reagent sc-735 X be used for Western Blot and other applications, or is it exclusively for use in GelShift and ChIP?
Asked by: TinTin
Thank you for your question. The TransCruz form of this antibody, sc-735 X, can be used in applications such as Western Blot or IF/IHC, the recommended dilution factor just needs to be scaled up by 10 due to the increased concentration of the vial.
Rated 2 out of
Faint band after western blotting.It detected a very faint band at the size expected for endogenous TAFII-p250 in lysates prepared from HEK 293T cells (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date published: 2018-04-09
Rated 1 out of
TAF1 antibodyI got free sample to test, and it works neither for western nor IP.
Date published: 2017-10-17
Rated 5 out of
Well characterized TAF II ab for ChIP and WBYour go to monoclonal ab for TAF II for ChIP and WB. With over 45 citations in the primary literature and strong in-house data this is a solid choice.
Date published: 2017-01-07
Rated 5 out of
Good WB publishable data gathered with HEpGood WB publishable data gathered with HEp-2 cells. -SCBT Publication Review
Date published: 2015-06-24
Rated 5 out of
no commentno comment
Date published: 2015-05-15
Rated 4 out of
This antibody worked well for IPThis antibody worked well for IP, published with MCF-7, U2OS and HCT116. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of
Desirable band using HeLa and ADesirable band using HeLa and A-431 whole cell lysates in Western Blot. -SCBT QC
Date published: 2015-05-02
Rated 5 out of
Published data for IP and WB in rat liverPublished data for IP and WB in rat liver extract -SCBT Publication Review
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