TAF II p18 Antibody (A-5): sc-393319
- TAF II p18 Antibody (A-5) is a mouse monoclonal IgG2b κ TAF II p18 antibody provided at 200 µg/ml
- raised against amino acids 1-124 representing full length TAF II p18 of human origin
- TAF II p18 Antibody (A-5) is recommended for detection of TAF II p18 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-TAF II p18 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for TAF II p18 Antibody (A-5) for WB applications. These reagents are now offered in bundles with TAF II p18 Antibody (A-5) (see ordering information below).
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TAF II p18 Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects TAF II p18 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAF II p18 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TAF II p18 plays a crucial role in assembling transcription factor TFIID, which is essential for preinitiation complex formation at the TATA promoter element. TFIID is a multisubunit complex composed of TATA-binding protein and several TBP-associated factors (TAFs), including TAF II p18, which belongs to a family that includes p28, p32, p100, p130, p170, and p250. TAF II p18′s interaction with other TAFs, particularly TAF II p28, is vital for transcription regulation, as these proteins work together to enhance various nuclear receptor activities, including estrogen and vitamin D3 receptors. TAF II p18 also modulates transcriptional response during cellular stress, highlighting TAF II p18′s importance in gene expression regulation and cellular homeostasis. Anti-TAF II p18 antibody (A-5)′s availability in multiple formats enables versatile research applications, making this antibody an invaluable tool for studying transcriptional regulation in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAF II p18 Antibody (A-5) References:
- Repression of A TAF(II)32 isoform as part of a program of genes regulated during mpl ligand-induced megakaryocyte differentiation. | Thompson, A. and Ravid, K. 1999. Biochem Biophys Res Commun. 262: 55-9. PMID: 10448067
- Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). | Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
- Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation. | Dynlacht, BD., et al. 1991. Cell. 66: 563-76. PMID: 1907890
- Five intermediate complexes in transcription initiation by RNA polymerase II. | Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
- Multiple factors required for accurate initiation of transcription by purified RNA polymerase II. | Matsui, T., et al. 1980. J Biol Chem. 255: 11992-6. PMID: 7440580
- Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation. | Klemm, RD., et al. 1995. Proc Natl Acad Sci U S A. 92: 5788-92. PMID: 7597030
- Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID. | Mengus, G., et al. 1995. EMBO J. 14: 1520-31. PMID: 7729427
- Human TAF(II28) promotes transcriptional stimulation by activation function 2 of the retinoid X receptors. | May, M., et al. 1996. EMBO J. 15: 3093-104. PMID: 8670810
- The class II trans-activator CIITA interacts with the TBP-associated factor TAFII32. | Fontes, JD., et al. 1997. Nucleic Acids Res. 25: 2522-8. PMID: 9171108
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF II p18 Antibody (A-5) | sc-393319 | 200 µg/ml | $322.00 | |||
TAF II p18 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-526239 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF II p18 Antibody (A-5): m-IgG2b BP-HRP Bundle | sc-549700 | 200 µg Ab; 10 µg BP | $361.00 | |||
TAF II p18 Antibody (A-5) AC | sc-393319 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TAF II p18 Antibody (A-5) HRP | sc-393319 HRP | 200 µg/ml | $322.00 | |||
TAF II p18 Antibody (A-5) FITC | sc-393319 FITC | 200 µg/ml | $336.00 | |||
TAF II p18 Antibody (A-5) PE | sc-393319 PE | 200 µg/ml | $349.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 488 | sc-393319 AF488 | 200 µg/ml | $364.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 546 | sc-393319 AF546 | 200 µg/ml | $364.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 594 | sc-393319 AF594 | 200 µg/ml | $364.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 647 | sc-393319 AF647 | 200 µg/ml | $364.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 680 | sc-393319 AF680 | 200 µg/ml | $364.00 | |||
TAF II p18 Antibody (A-5) Alexa Fluor® 790 | sc-393319 AF790 | 200 µg/ml | $364.00 |