SYP/Synaptophysin Antibody (C-12): sc-365447
- SYP/Synaptophysin Antibody (C-12) is a mouse monoclonal IgG1, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 289-312 at the C-terminus of SYP of human origin
- recommended for detection of SYP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See SYP/Synaptophysin (D-4): sc-17750 for SYP/Synaptophysin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SYP/Synaptophysin Antibody (C-12) for WB and IHC(P) applications. These reagents are now offered in bundles with SYP/Synaptophysin Antibody (C-12) (see ordering information below).
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SYP Antibody (C-12) is a mouse monoclonal IgG1 antibody that detects synaptophysin (SYP) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SYP is an integral membrane protein predominantly found in neurons and endocrine cells, playing a crucial role in the formation and recycling of synaptic vesicles. SYP is characterized by N-glycosylation and the ability to form hexameric complexes, which are essential for function as a large conductance channel. SYP contains four transmembrane domains, which are vital for structural integrity and functionality, allowing facilitation of neurotransmitter transport. SYP interacts with membrane cholesterol, which is critical for vesicle formation, and forms complexes with synaptobrevin (VAMP) that are upregulated during neuronal development. While the synaptophysin-synaptobrevin complex is not essential for the exocytosis process, this complex serves as a reservoir of synaptobrevin, ensuring sufficient levels are available for neurotransmitter release. Additionally, the tail domain of brain Myosin V forms a stable complex with synaptobrevin II and SYP, which disassembles upon calcium influx during neuronal depolarization, highlighting the dynamic nature of these interactions in synaptic function. SYP Antibody (C-12) serves as an invaluable tool for researchers studying synaptic mechanisms and synaptophysin′s role in neurobiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SYP/Synaptophysin Antibody (C-12) References:
- Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats. | Zhu, X., et al. 2019. Int J Med Sci. 16: 394-402. PMID: 30911273
- Synaptophysin Regulates Fusion Pores and Exocytosis Mode in Chromaffin Cells. | Chang, CW., et al. 2021. J Neurosci. 41: 3563-3578. PMID: 33664131
- Synaptophysin controls synaptobrevin-II retrieval via a cryptic C-terminal interaction site. | Harper, CB., et al. 2021. J Biol Chem. 296: 100266. PMID: 33769286
- Synaptophysin and chromogranin A expression analysis in human tumors. | Uhlig, R., et al. 2022. Mol Cell Endocrinol. 555: 111726. PMID: 35921917
- Synaptophysin transmembrane domain III controls fusion pore dynamics in Ca2+-triggered exocytosis. | Hsiao, YT. and Jackson, MB. 2023. Biophys J. 122: 1962-1973. PMID: 36168290
- Aberrant synaptophysin expression in classic Hodgkin lymphoma. | Im, S., et al. 2022. Diagn Pathol. 17: 90. PMID: 36401284
- Circ-Vps41 positively modulates Syp and its overexpression improves memory ability in aging mice. | Li, Y., et al. 2022. Front Mol Neurosci. 15: 1037912. PMID: 36533129
- Sequences of synaptogenesis in the human fetal and neonatal brain by synaptophysin immunocytochemistry. | Sarnat, HB. 2023. Front Cell Neurosci. 17: 1105183. PMID: 36816854
- Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles. | Park, D., et al. 2024. Proc Natl Acad Sci U S A. 121: e2409605121. PMID: 38985768
- Absence of ATG9A and synaptophysin demixing on Rab5 mutation-induced giant endosomes. | Choi, J., et al. 2024. Mol Brain. 17: 63. PMID: 39223639
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SYP/Synaptophysin Antibody (C-12) | sc-365447 | 200 µg/ml | $322.00 | |||
SYP/Synaptophysin Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-537803 | 200 µg Ab; 10 µg BP | $361.00 | |||
SYP/Synaptophysin Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-535199 | 200 µg Ab; 40 µg BP | $361.00 | |||
SYP/Synaptophysin Antibody (C-12): m-IgG1 BP-HRP Bundle | sc-545365 | 200 µg Ab; 20 µg BP | $361.00 | |||
SYP/Synaptophysin (C-12) Neutralizing Peptide | sc-365447 P | 100 µg/0.5 ml | $69.00 |