Date published: 2026-4-24
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symplekin Antibody (E-2) - Western Blotting - Image 318355
symplekin Antibody (E-2): sc-514260. Western blot analysis of symplekin expression in COLO 320DM (A), Jurkat (B) and K-562 (C) whole cell lysates.
symplekin Antibody (E-2) - Western Blotting - Image 318355
symplekin Antibody (E-2): sc-514260. Western blot analysis of symplekin expression in COLO 320DM (A), Jurkat (B) and K-562 (C) whole cell lysates.
symplekin Antibody (E-2) - Western Blotting - Image 371337
symplekin Antibody (E-2): sc-514260. Western blot analysis of symplekin expression in K-562 (A), Caco-2 (B), MCF7 (C) and NIH/3T3 (D) whole cell lysates.
symplekin Antibody (E-2): sc-514260. Western blot analysis of symplekin expression in COLO 320DM (A), Jurkat (B) and K-562 (C) whole cell lysates.

symplekin Antibody (E-2): sc-514260

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Datasheets
  • symplekin Antibody (E-2) is a mouse monoclonal mouse IgG1 κ κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 517-816 mapping within an internal region of symplekin of human origin
  • recommended for detection of symplekin of mouse, rat and human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-514260 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for symplekin Antibody (E-2) for WB applications. These reagents are now offered in bundles with symplekin Antibody (E-2) (see ordering information below).
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SEE ALSO...

Symplekin Antibody (E-2) is a mouse monoclonal IgG1 antibody that detects symplekin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Symplekin is a crucial regulatory protein that plays a significant role in the polyadenylation of mRNA, which is essential for gene expression and the stability of mRNA molecules. Symplekin is predominantly located in the nucleus, cytoplasm, cytoskeleton, and cell junctions, highlighting versatile functions within the cell. Symplekin forms a high molecular weight complex, known as the tight junction complex, with other proteins involved in polyadenylation, underscoring importance in recruiting regulatory proteins to specific sites for efficient gene expression. Symplekin is involved in the 3′-end maturation of histone mRNAs, which is vital for proper cell cycle regulation and gene transcription. Defects in the gene encoding symplekin have been linked to hepatocellular carcinoma, indicating potential role in tumorigenesis. Symplekin is expressed as two isoforms due to alternative splicing, which may contribute to diverse functional roles in various tissues, including the testis, pancreas, stomach, liver, and fetal brain.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

symplekin Antibody (E-2) References:

  1. Complex protein interactions within the human polyadenylation machinery identify a novel component.  |  Takagaki, Y. and Manley, JL. 2000. Mol Cell Biol. 20: 1515-25. PMID: 10669729
  2. HSF1 modulation of Hsp70 mRNA polyadenylation via interaction with symplekin.  |  Xing, H., et al. 2004. J Biol Chem. 279: 10551-5. PMID: 14707147
  3. Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin.  |  Kavanagh, E., et al. 2006. J Cell Sci. 119: 5098-105. PMID: 17158914
  4. Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex.  |  Vethantham, V., et al. 2007. Mol Cell Biol. 27: 8848-58. PMID: 17923699
  5. Protein factors in pre-mRNA 3'-end processing.  |  Mandel, CR., et al. 2008. Cell Mol Life Sci. 65: 1099-122. PMID: 18158581
  6. A core complex of CPSF73, CPSF100, and Symplekin may form two different cleavage factors for processing of poly(A) and histone mRNAs.  |  Sullivan, KD., et al. 2009. Mol Cell. 34: 322-32. PMID: 19450530
  7. Crystal structure of the HEAT domain from the Pre-mRNA processing factor Symplekin.  |  Kennedy, SA., et al. 2009. J Mol Biol. 392: 115-28. PMID: 19576221
  8. Drosophila melanogaster retrotransposon and inverted repeat-derived endogenous siRNAs are differentially processed in distinct cellular locations.  |  Harrington, AW., et al. 2017. BMC Genomics. 18: 304. PMID: 28415970
  9. Chromosomal localization to 19q13.3, partial genomic structure and 5' cDNA sequence of the human symplekin gene.  |  Ueki, K., et al. 1997. Somat Cell Mol Genet. 23: 229-31. PMID: 9330635
  10. Molecular architecture of tight junctions.  |  Mitic, LL. and Anderson, JM. 1998. Annu Rev Physiol. 60: 121-42. PMID: 9558457

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

symplekin Antibody (E-2)

sc-514260
200 µg/ml
$322.00

symplekin Antibody (E-2): m-IgG Fc BP-HRP Bundle

sc-538611
200 µg Ab; 10 µg BP
$361.00

symplekin Antibody (E-2): m-IgGκ BP-HRP Bundle

sc-536255
200 µg Ab; 40 µg BP
$361.00

symplekin Antibody (E-2): m-IgG1 BP-HRP Bundle

sc-545793
200 µg Ab; 20 µg BP
$361.00

symplekin Antibody (E-2) X

sc-514260 X
200 µg/0.1 ml
$322.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if symplekin (E-2): sc-514260 mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Works for Western BlottingI tried this antibody for Western blotting with 1:100 dilution. The band I can detect is clear and specific, but relatively weak, may need to increase the antibody concentration for stronger signal.
Date published: 2017-11-15
Rated 5 out of 5 by from Great Western blot data of symplekin expressionGreat Western blot data of symplekin expression in COLO 320DM, Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2014-10-23
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symplekin Antibody (E-2) is rated 4.5 out of 5 by 2.
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