Syk Antibody (D-3): sc-166226
- Syk Antibody (D-3) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 11-41 at the N-terminus of Syk of human origin
- Anti-Syk Antibody (D-3) is recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- Anti-Syk Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Syk Antibody (D-3) for WB applications. These reagents are now offered in bundles with Syk Antibody (D-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
Syk Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Syk in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Syk (D-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Syk, or spleen tyrosine kinase, is a 635 amino acid protein characterized by one protein kinase domain and two SH2 domains, playing a crucial role in B cell signaling. Specifically, Syk acts as a positive effector in B cell antigen receptor (CD79)-stimulated responses, facilitating calcium ion movement through two distinct phospho-regulated pathways. When Syk is unphosphorylated, calcium ions travel via a phosphoinositide 3-kinase-dependent pathway, while phosphorylation at tyrosine residues 348 and 352 shifts calcium flow to a phospholipase C γ-dependent pathway. This regulation of calcium movement is vital for various cellular processes, including differentiation, phagocytosis, proliferation, and B cell development. Notably, Syk expression is upregulated in T cell lymphoma, indicating potential involvement in tumorigenesis. Additionally, Syk exists in two isoforms, short and long, resulting from alternative splicing events, which may contribute to functional diversity in cellular signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Syk Antibody (D-3) References:
- Tyrosine kinase SYK: essential functions for immunoreceptor signalling. | Turner, M., et al. 2000. Immunol Today. 21: 148-54. PMID: 10689303
- CARD9 Syk-dependent and Raf-1 Syk-independent signaling pathways in target recognition of Candida albicans by Dectin-1. | Yang, H., et al. 2011. Eur J Clin Microbiol Infect Dis. 30: 303-5. PMID: 21108038
- Syk Is Recruited to Stress Granules and Promotes Their Clearance through Autophagy. | Krisenko, MO., et al. 2015. J Biol Chem. 290: 27803-15. PMID: 26429917
- Feedback Regulation of Syk by Protein Kinase C in Human Platelets. | Makhoul, S., et al. 2019. Int J Mol Sci. 21: PMID: 31881809
- Regulatory interplay between Vav1, Syk and β-catenin occurs in lung cancer cells. | Boudria, R., et al. 2021. Cell Signal. 86: 110079. PMID: 34252536
- Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia. | Singaram, I., et al. 2023. Nat Chem Biol. 19: 239-250. PMID: 36229686
- Orientia tsutsugamushi Infection Stimulates Syk-Dependent Responses and Innate Cytosolic Defenses in Macrophages. | Fisher, J., et al. 2022. Pathogens. 12: PMID: 36678402
- Differential Regulation of GPVI-Induced Btk and Syk Activation by PKC, PKA and PP2A in Human Platelets. | Zhang, P., et al. 2023. Int J Mol Sci. 24: PMID: 37175486
- Biochemical characterization of spleen tyrosine kinase (SYK) isoforms in platelets. | Kumar Singh, P., et al. 2023. Platelets. 34: 2249549. PMID: 37661351
- SIRPB1 regulates inflammatory factor expression in the glioma microenvironment via SYK: functional and bioinformatics insights. | Geng, R., et al. 2024. J Transl Med. 22: 338. PMID: 38594692
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Syk Antibody (D-3) | sc-166226 | 200 µg/ml | $322.00 | |||
Syk Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-528925 | 200 µg Ab; 10 µg BP | $361.00 | |||
Syk Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-521539 | 200 µg Ab, 40 µg BP | $361.00 | |||
Syk Antibody (D-3): m-IgG1 BP-HRP Bundle | sc-543215 | 200 µg Ab; 20 µg BP | $361.00 | |||
Syk Antibody (D-3) AC | sc-166226 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Syk Antibody (D-3) HRP | sc-166226 HRP | 200 µg/ml | $322.00 | |||
Syk Antibody (D-3) FITC | sc-166226 FITC | 200 µg/ml | $336.00 | |||
Syk Antibody (D-3) PE | sc-166226 PE | 200 µg/ml | $349.00 | |||
Syk Antibody (D-3) Alexa Fluor® 488 | sc-166226 AF488 | 200 µg/ml | $364.00 | |||
Syk Antibody (D-3) Alexa Fluor® 546 | sc-166226 AF546 | 200 µg/ml | $364.00 | |||
Syk Antibody (D-3) Alexa Fluor® 594 | sc-166226 AF594 | 200 µg/ml | $364.00 | |||
Syk Antibody (D-3) Alexa Fluor® 647 | sc-166226 AF647 | 200 µg/ml | $364.00 | |||
Syk Antibody (D-3) Alexa Fluor® 680 | sc-166226 AF680 | 200 µg/ml | $364.00 | |||
Syk Antibody (D-3) Alexa Fluor® 790 | sc-166226 AF790 | 200 µg/ml | $364.00 | |||
Syk (D-3) Neutralizing Peptide | sc-166226 P | 100 µg/0.5 ml | $69.00 |