Syk Antibody (4D10) recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Syk Antibody (4D10): sc-1240

Syk Antibody (4D10) is rated 4.7 out of 5 by 17.
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Datasheets

    • Anti-Syk Antibody (4D10) is a mouse monoclonal IgG2a (kappa light chain) Syk antibody provided at 200 µg/ml
    • raised against amino acids 313-339 of Syk of human origin
    • Anti-Syk Antibody (4D10) is recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-Syk Antibody (4D10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Syk (4D10): sc-1240.

How to grade the positivity in breast carcinoma cells

Asked by: Ghazala
Thank you for your question. You would require appropriate positive and negative controls to compare the signals between different samples. Recommended positive controls are NAMALWA cell lysate: sc-2234, BJAB whole cell lysate: sc-2207 or Raji whole cell lysate: sc-364236. We also recommend using direct conjugates for detection so that signal strength directly corresponds to the amount of protein present.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Is it possible for the Syk Antibody (4D10): sc-1240 to react with monkey tissue samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on the webpage, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function. We will be more than happy to run sequence analysis for your species of interest.
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 4 out of 5 by from Works good! Good a free sample to test. Planing to use in future! For WB perferct!
Date published: 2018-02-13
Rated 5 out of 5 by from Great antibody for western blot The batch of antibody I have worked well on HeLa, NIH/3T3, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2017-11-16
Rated 5 out of 5 by from The antibody worked in western blot This antibody worked very well in western blot on mouse osteoclasts extract. We diluted it at 1/200 but it could be diluted more because the signal was strong.
Date published: 2017-10-25
Rated 5 out of 5 by from It works great. I tested in PC3 cells. It worked well at 1:200 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from worked well. I got this antibody as free samples and it worked very well for WB. I used 1:500 dilution.
Date published: 2017-03-15
Rated 5 out of 5 by from Excellent antibody for westerns Probed this antibody at 1/1000 dilution for one hour and used the Kappa-binding HRP as secondary: the result was flawless. Excellent antibody for westerns.
Date published: 2017-03-08
Rated 5 out of 5 by from Ottima reattività Utilizzato per rilevare la chinasi Syk in cellule Natural Killer primarie. Ottima reattività sia su whole cell lysates che in immunoprecipitazione.
Date published: 2017-02-06
Rated 5 out of 5 by from Well published Many citations using this antibody in a variety of applications, especially Western Blot and IP!
Date published: 2016-12-27
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