SWAP-70 Antibody (F-3): sc-390431
- SWAP-70 Antibody (F-3) is a mouse monoclonal IgG1 κ SWAP-70 antibody provided at 200 µg/ml
- raised against amino acids 455-585 mapping at the C-terminus of SWAP-70 of human origin
- SWAP-70 Antibody (F-3) is recommended for detection of SWAP-70 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SWAP-70 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SWAP-70 Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with SWAP-70 Antibody (F-3) (see ordering information below).
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SWAP-70 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects SWAP-70 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SWAP-70 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SWAP-70 protein plays a crucial role in immune response, particularly in B cell activation and differentiation. SWAP-70 is involved in forming a protein complex that facilitates cell-free DNA recombination between immunoglobulin heavy chain gene switch region substrates, which is essential for generating diverse antibody responses. SWAP-70 is primarily localized in resting B lymphocyte cytoplasm, but upon activation, translocates to plasma membrane and subsequently to nucleus, where SWAP-70 recognizes specific switch regions and acts as a switch recombinase, inducing DNA breaks. This dynamic localization underscores SWAP-70′s importance in signaling pathways during B cell activation. SWAP-70 contains three nuclear localization signals, exhibits weak DNA affinity, binds ATP, and forms high-affinity complexes with proteins such as B23, C23, and poly (ADP-ribose) polymerase, further highlighting SWAP-70′s multifaceted role in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SWAP-70 Antibody (F-3) References:
- Cellular, intracellular, and developmental expression patterns of murine SWAP-70. | Borggrefe, T., et al. 1999. Eur J Immunol. 29: 1812-22. PMID: 10382743
- Mapping of the SWAP70 gene to mouse chromosome 7 and human chromosome 11p15. | Masat, L., et al. 2000. Immunogenetics. 51: 16-9. PMID: 10663557
- Association of SWAP-70 with the B cell antigen receptor complex. | Masat, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 2180-4. PMID: 10681448
- Differential regulation of germinal center genes, BCL6 and SWAP-70, during the course of MAIDS. | Qi, CF., et al. 1999. Mol Immunol. 36: 1043-53. PMID: 10698307
- A B-cell-specific DNA recombination complex. | Borggrefe, T., et al. 1998. J Biol Chem. 273: 17025-35. PMID: 9642267
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SWAP-70 Antibody (F-3) | sc-390431 | 200 µg/ml | $322.00 | |||
SWAP-70 Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-530157 | 200 µg Ab; 10 µg BP | $361.00 | |||
SWAP-70 Antibody (F-3): m-IgGκ BP-HRP Bundle | sc-523533 | 200 µg Ab, 40 µg BP | $361.00 | |||
SWAP-70 Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-543900 | 200 µg Ab; 20 µg BP | $361.00 | |||
SWAP-70 Antibody (F-3) AC | sc-390431 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SWAP-70 Antibody (F-3) HRP | sc-390431 HRP | 200 µg/ml | $322.00 | |||
SWAP-70 Antibody (F-3) FITC | sc-390431 FITC | 200 µg/ml | $336.00 | |||
SWAP-70 Antibody (F-3) PE | sc-390431 PE | 200 µg/ml | $349.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 488 | sc-390431 AF488 | 200 µg/ml | $364.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 546 | sc-390431 AF546 | 200 µg/ml | $364.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 594 | sc-390431 AF594 | 200 µg/ml | $364.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 647 | sc-390431 AF647 | 200 µg/ml | $364.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 680 | sc-390431 AF680 | 200 µg/ml | $364.00 | |||
SWAP-70 Antibody (F-3) Alexa Fluor® 790 | sc-390431 AF790 | 200 µg/ml | $364.00 |