SUG1 Antibody (C-3): sc-376389

SUG1 Antibody (C-3) is a mouse monoclonal IgG1 antibody that detects SUG1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SUG1, also known as PSMC5, is a crucial ATPase subunit of the 26S proteasome complex, which plays a vital role in the regulated degradation of ubiquitinated proteins, thereby maintaining cellular homeostasis and regulating various signaling pathways. SUG1 is primarily localized in the cytoplasm and nucleus, where SUG1 is integral to the 19S regulatory particle of the proteasome, facilitating the recognition and processing of substrates, particularly those involved in endoplasmic reticulum-associated degradation. SUG1′s importance extends beyond a structural role; recent research indicates that SUG1 phosphorylation by specific protein kinases is essential for the assembly of the 26S proteasome, highlighting SUG1′s regulatory function in proteasome biogenesis. Furthermore, SUG1 has been shown to interact with key transcription factors such as the retinoid X receptor and thyroid hormone receptor, suggesting that SUG1 may also play a significant role in modulating gene expression in response to hormonal signals. With these multifaceted functions, anti-SUG1 antibody (C-3) serves as an invaluable tool for researchers studying proteasome dynamics and related signaling pathways.

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SUG1 Antibody (C-3) References:

1. Phosphorylation by p38MAPK and recruitment of SUG-1 are required for RA-induced RAR gamma degradation and transactivation.  |  Giannì, M., et al. 2002. EMBO J. 21: 3760-9. PMID: 12110588
2. Mouse homologue of yeast Prp19 interacts with mouse SUG1, the regulatory subunit of 26S proteasome.  |  Sihn, CR., et al. 2007. Biochem Biophys Res Commun. 356: 175-80. PMID: 17349974
3. The 19S proteasome ATPase Sug1 plays a critical role in regulating MHC class II transcription.  |  Bhat, KP., et al. 2008. Mol Immunol. 45: 2214-24. PMID: 18215421
4. SUG-1 plays proteolytic and non-proteolytic roles in the control of retinoic acid target genes via its interaction with SRC-3.  |  Ferry, C., et al. 2009. J Biol Chem. 284: 8127-35. PMID: 19144644
5. Interaction with Sug1 enables Ipaf ubiquitination leading to caspase 8 activation and cell death.  |  Kumar, Y., et al. 2010. Biochem J. 427: 91-104. PMID: 20085538
6. Role of Sug1, a 19S proteasome ATPase, in the transcription of MHC I and the atypical MHC II molecules, HLA-DM and HLA-DO.  |  Inostroza-Nieves, Y., et al. 2012. Immunol Lett. 147: 67-74. PMID: 22771340
7. Hypoxia-induced increase in Sug1 leads to poor post-transplantation survival of allogeneic mesenchymal stem cells.  |  Abu-El-Rub, E., et al. 2020. FASEB J. 34: 12860-12876. PMID: 32770803
8. SUG1, a putative transcriptional mediator and subunit of the PA700 proteasome regulatory complex, is a DNA helicase.  |  Fraser, RA., et al. 1997. J Biol Chem. 272: 7122-6. PMID: 9054406
9. SUG1, a component of the 26 S proteasome, is an ATPase stimulated by specific RNAs.  |  Makino, Y., et al. 1997. J Biol Chem. 272: 23201-5. PMID: 9287326
10. Interaction of phosducin and phosducin isoforms with a 26S proteasomal subunit, SUG1.  |  Zhu, X. and Craft, CM. 1998. Mol Vis. 4: 13. PMID: 9701609

The preferred SUG1 antibody is SUG1 Antibody (H-7): sc-390631.