Sucrase-Isomaltase Antibody (C-8): sc-393470

Sucrase-Isomaltase Antibody (C-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sucrase-Isomaltase in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Sucrase-Isomaltase (C-8) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. Sucrase-Isomaltase (SI) is a crucial type II brush border membrane protein located in the apical membrane of intestinal enterocytes, where SI plays a vital role in the final stages of carbohydrate digestion. SI functions as a disaccharidase, catalyzing the hydrolysis of dietary sucrose and maltose, which are essential for breaking down carbohydrates into absorbable monosaccharides. SI′s location is significant due to involvement in nutrient absorption; any deficiency can lead to severe digestive issues, such as osmotic diarrhea, due to the inability to properly hydrolyze disaccharides. Congenital Sucrase-Isomaltase deficiency (CSID) is an autosomal recessive disorder that highlights SI′s importance, as CSID results in reduced enzymatic activity and subsequent malabsorption of carbohydrates, emphasizing SI′s critical role in maintaining digestive health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sucrase-Isomaltase Antibody (C-8) References:

1. Brush border membrane sucrase-isomaltase, maltase-glucoamylase and trehalase in mammals. Comparative development, effects of glucocorticoids, molecular mechanisms, and phylogenetic implications.  |  Galand, G. 1989. Comp Biochem Physiol B. 94: 1-11. PMID: 2513162
2. Molecular pathogenicity of novel sucrase-isomaltase mutations found in congenital sucrase-isomaltase deficiency patients.  |  Gericke, B., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 817-826. PMID: 28062276
3. Phylogenetic analysis reveals key residues in substrate hydrolysis in the isomaltase domain of sucrase-isomaltase and its role in starch digestion.  |  Chaudet, MM., et al. 2019. Biochim Biophys Acta Gen Subj. 1863: 1410-1416. PMID: 31254546
4. Sucrase-Isomaltase Deficiency as a Potential Masquerader in Irritable Bowel Syndrome.  |  Kim, SB., et al. 2020. Dig Dis Sci. 65: 534-540. PMID: 31493040
5. Loss of Sucrase-Isomaltase Function Increases Acetate Levels and Improves Metabolic Health in Greenlandic Cohorts.  |  Andersen, MK., et al. 2022. Gastroenterology. 162: 1171-1182.e3. PMID: 34914943
6. Sucrase-isomaltase genotype and response to a starch-reduced and sucrose-reduced diet in IBS-D patients.  |  Zamfir-Taranu, A., et al. 2024. Gut. 73: 706-708. PMID: 36878682
7. Impaired digestive function of sucrase-isomaltase in a complex with the Greenlandic sucrase-isomaltase variant.  |  Tannous, S. and Naim, HY. 2024. Biochim Biophys Acta Mol Basis Dis. 1870: 166947. PMID: 37951511
8. Sucrase-isomaltase deficiency-a frequently misdiagnosed disease.  |  Ament, ME., et al. 1973. J Pediatr. 83: 721-7. PMID: 4742566
9. Sucrase-isomaltase: a stalked intrinsic protein of the brush border membrane.  |  Hauser, H. and Semenza, G. 1983. CRC Crit Rev Biochem. 14: 319-45. PMID: 6357634
10. Human intestinal sucrase-isomaltase. Identification of free sucrase and isomaltase and cleavage of the hybrid into active distinct subunits.  |  Conklin, KA., et al. 1975. J Biol Chem. 250: 5735-41. PMID: 807575

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sucrase-Isomaltase Antibody (C-8) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Sucrase-Isomaltase Antibody (C-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sucrase-Isomaltase Antibody (C-8) Specifications

Alternate Product Name: Sucrase-Isomaltase

Product Clone Name: C-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 136-167

Epitope Target: Sucrase-Isomaltase

Epitope Species: rat

Product Reactivity/Detection: precursor and mature isomaltase isoform of Sucrase-Isomaltase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Sucrase-Isomaltase Antibody (C-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Sucrase-Isomaltase antibody is Sucrase-Isomaltase Antibody (A-12): sc-393424.