STEP Antibody (F-9): sc-514678

STEP Antibody (F-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects STEP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-STEP antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. STEP, or striatal enriched phosphatase, is a member of a brain-specific family of protein tyrosine phosphatases that play a crucial role in synaptic signaling and plasticity. STEP is predominantly expressed in dopaminoceptive neurons within the central nervous system, particularly in the basal ganglia, where STEP regulates the N-methyl-d-aspartate receptor (NMDAR) complex. STEP is essential for modulating synaptic transmission, as STEP depresses both NMDAR single-channel activity and synaptic currents, thereby influencing neuronal communication and plasticity. The membrane-associated STEP61 isoform, which contains a single tyrosine phosphatase domain, two proline-rich domains, and two transmembrane domains, is localized in the postsynaptic densities of striatal neurons. STEP61 interacts with the Src family kinase member Fyn, specifically when Fyn is phosphorylated at Tyr 420, allowing STEP61 to dephosphorylate this residue and potentially regulate Fyn activity within the postsynaptic density. The STEP20 isoform, isolated from mouse brain, lacks the conserved tyrosine phosphatase domain, highlighting the diversity within the STEP family. The human STEP gene is located on chromosome 11p15.2-p15.1, underscoring its evolutionary conservation and significance in neurobiology.

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STEP Antibody (F-9) References:

1. Genetic reduction of striatal-enriched tyrosine phosphatase (STEP) reverses cognitive and cellular deficits in an Alzheimer's disease mouse model.  |  Zhang, Y., et al. 2010. Proc Natl Acad Sci U S A. 107: 19014-9. PMID: 20956308
2. Striatal-enriched protein tyrosine phosphatase expression and activity in Huntington's disease: a STEP in the resistance to excitotoxicity.  |  Saavedra, A., et al. 2011. J Neurosci. 31: 8150-62. PMID: 21632937
3. Therapeutic implications for striatal-enriched protein tyrosine phosphatase (STEP) in neuropsychiatric disorders.  |  Goebel-Goody, SM., et al. 2012. Pharmacol Rev. 64: 65-87. PMID: 22090472
4. Calpain and STriatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington's disease mouse model.  |  Gladding, CM., et al. 2012. Hum Mol Genet. 21: 3739-52. PMID: 22523092
5. Behavioral characterization of striatal-enriched protein tyrosine phosphatase (STEP) knockout mice.  |  Sukoff Rizzo, SJ., et al. 2014. Genes Brain Behav. 13: 643-52. PMID: 25103464
6. Disruption of striatal-enriched protein tyrosine phosphatase (STEP) function in neuropsychiatric disorders.  |  Karasawa, T. and Lombroso, PJ. 2014. Neurosci Res. 89: 1-9. PMID: 25218562
7. Striatal-enriched Tyrosine Protein Phosphatase (STEP) in the Mechanisms of Depressive Disorders.  |  Kulikova, E. and Kulikov, A. 2017. Curr Protein Pept Sci. 18: 1152-1162. PMID: 28699511
8. On an association between fear-induced aggression and striatal-enriched protein tyrosine phosphatase (STEP) in the brain of Norway rats.  |  Moskaliuk, VS., et al. 2022. Biomed Pharmacother. 147: 112667. PMID: 35104695
9. Detection of Cellular Target Engagement for Small-Molecule Modulators of Striatal-Enriched Protein Tyrosine Phosphatase (STEP).  |  Han, YN., et al. 2023. Methods Mol Biol. 2706: 167-175. PMID: 37558948
10. Design and synthesis of novel benzoic acid derivatives as striatal-enriched protein tyrosine phosphatase (STEP) inhibitors with neuroprotective properties.  |  Jiang, C., et al. 2025. Eur J Med Chem. 283: 117135. PMID: 39657460

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STEP Antibody (F-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-STEP Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STEP Antibody (F-9) Specifications

Alternate Product Name: STEP

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 197-240

Epitope Target: STEP

Epitope Species: human

Product Reactivity/Detection: STEP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for STEP Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred STEP antibody is STEP Antibody (23E5): sc-23892.