STEP Antibody (23E5): sc-23892

STEP Antibody (23E5) is a mouse monoclonal IgG2b kappa light chain antibody raised against an 18 amino acid sequence mapping at the N-terminus of STEP46 of rat origin. STEP Antibody (23E5) detects STEP protein of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. STEP Antibody (23E5) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. STEP (striatal enriched phosphatase) belongs to the brain-specific family of protein tyrosine phosphatases and regulates the N-methyl-D-aspartate receptor (NMDAR) complex, essential for synaptic plasticity and memory formation. STEP modulates NMDAR activity, influences synaptic transmission and neuronal signaling, supporting proper cognitive function. STEP is predominantly expressed in dopaminoceptive neurons within the central nervous system, particularly in the basal ganglia, where STEP regulates synaptic currents and receptor activity. STEP61 isoform, containing a single tyrosine phosphatase domain, two proline-rich domains, and two transmembrane domains, localizes in postsynaptic densities of striatal neurons. STEP61 interacts with Src family kinase member Fyn when phosphorylated at Tyr 420, enabling STEP61 to dephosphorylate this residue and modulate Fyn activity within the postsynaptic density. STEP20 isoform, isolated from mouse brain, lacks the conserved tyrosine phosphatase domain. STEP gene is located on human chromosome 11p15.2-p15.1. STEP Antibody (23E5) enables researchers to detect and analyze STEP protein expression and interactions, advancing the understanding of synaptic regulation and molecular basis of neurological disorders.

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STEP Antibody (23E5) References:

1. Overexpression of striatal enriched phosphatase (STEP) promotes the neurite outgrowth induced by a cAMP analogue in PC12 cells.  |  Okamura, A., et al. 1999. Brain Res Mol Brain Res. 67: 1-9. PMID: 10101226
2. Expression of neuron specific phosphatase, striatal enriched phosphatase (STEP) in reactive astrocytes after transient forebrain ischemia.  |  Hasegawa, S., et al. 2000. Glia. 29: 316-29. PMID: 10652442
3. The Dopamine/D1 receptor mediates the phosphorylation and inactivation of the protein tyrosine phosphatase STEP via a PKA-dependent pathway.  |  Paul, S., et al. 2000. J Neurosci. 20: 5630-8. PMID: 10908600
4. Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation.  |  Pelkey, KA., et al. 2002. Neuron. 34: 127-38. PMID: 11931747
5. Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420.  |  Nguyen, TH., et al. 2002. J Biol Chem. 277: 24274-9. PMID: 11983687
6. Striatal-enriched phosphatase 61 inhibited the nociceptive plasticity in spinal cord dorsal horn of rats.  |  Suo, ZW., et al. 2017. Neuroscience. 352: 97-105. PMID: 28389375
7. Alterations of STEP46 and STEP61 Expression in the Rat Retina with Age and AMD-Like Retinopathy Development.  |  Telegina, DV., et al. 2020. Int J Mol Sci. 21: PMID: 32707818
8. Molecular cloning of the human homolog of a striatum-enriched phosphatase (STEP) gene and chromosomal mapping of the human and murine loci.  |  Li, X., et al. 1995. Genomics. 28: 442-9. PMID: 7490079
9. Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases.  |  Sharma, E., et al. 1995. Brain Res Mol Brain Res. 32: 87-93. PMID: 7494467
10. A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures.  |  Lombroso, PJ., et al. 1993. J Neurosci. 13: 3064-74. PMID: 8331384
11. STEP: a family of brain-enriched PTPs. Alternative splicing produces transmembrane, cytosolic and truncated isoforms.  |  Bult, A., et al. 1997. Eur J Cell Biol. 72: 337-44. PMID: 9127733
12. PTP-SL and STEP protein tyrosine phosphatases regulate the activation of the extracellular signal-regulated kinases ERK1 and ERK2 by association through a kinase interaction motif.  |  Pulido, R., et al. 1998. EMBO J. 17: 7337-50. PMID: 9857190

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-STEP Antibody (23E5) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-STEP Antibody (23E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

STEP Antibody (23E5) Specifications

Alternate Product Name: STEP

Product Clone Name: 23E5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: STEP46

Epitope Species: rat

Product Reactivity/Detection: all known isoforms of STEP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS containing 0.1% sodium azide and 0.2% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for STEP Antibody (23E5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred STEP antibody is STEP Antibody (F-9): sc-514678.