Stat4 Antibody (C-4): sc-398228

Stat4 Antibody (C-4) is a mouse monoclonal IgG2b kappa light chain antibody designed to detect Signal Transducer and Activator of Transcription 4 (STAT4), a crucial member of the STAT family involved in mediating cytokine signaling and immune response regulation. STAT4 monoclonal antibody (C-4) targets an epitope mapping between amino acids 10-31 at the N-terminus of mouse STAT4, ensuring robust recognition across multiple species including mouse, rat, human, as well as equine, canine, bovine, porcine, and avian. STAT4 plays a pivotal role in the differentiation of naive T cells into Th1 cells by responding to cytokines like interleukin-12 (IL-12) and interleukin-23 (IL-23). Nuclear localization of STAT4 is essential for function as a transcription factor, where STAT4 interacts with Janus kinases (JAKs) for phosphorylation and activation, as well as with transcriptional co-activators like CBP/p300 to regulate gene expression. STAT4 forms complexes with other transcription factors, including STAT1 and STAT3, to modulate the expression of genes involved in immune responses, inflammation, and cell survival. Anti-STAT4 antibody (C-4) is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), and is available in both non-conjugated and multiple conjugated forms such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. STAT4 (C-4) antibody is an indispensable tool for researchers investigating molecular mechanisms of immune cell differentiation, cytokine signaling pathways, and the interplay between STAT4 and other regulatory proteins. Anti-STAT4 antibody (C-4) provides reliable and precise detection of STAT4 for studying autoimmune diseases, infectious responses, and cancer immunology, facilitating significant advancements in understanding and manipulating immune functions for therapeutic purposes.

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Stat4 Antibody (C-4) References:

1. Targeting of the transcription factor STAT4 by antisense phosphorothioate oligonucleotides suppresses collagen-induced arthritis.  |  Hildner, KM., et al. 2007. J Immunol. 178: 3427-36. PMID: 17339437
2. STAT4: genetics, mechanisms, and implications for autoimmunity.  |  Korman, BD., et al. 2008. Curr Allergy Asthma Rep. 8: 398-403. PMID: 18682104
3. Polymorphisms of the STAT4 gene in the pathogenesis of tuberculosis.  |  Wu, S., et al. 2018. Biosci Rep. 38: PMID: 30054428
4. Early antigen receptor signaling in natural killer cells alters STAT4-dependent fate decisions via epigenetic remodeling.  |  Grassmann, S., et al. 2023. bioRxiv.. PMID: 37986752
5. Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3.  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
6. Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3.  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
7. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
8. Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells.  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
9. Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation.  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
10. An interleukin-4-induced transcription factor: IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
11. Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
12. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Stat4 Antibody (C-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Stat4 Antibody (C-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Stat4 Antibody (C-4) Specifications

Alternate Product Name: Stat4

Product Clone Name: C-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 10-31

Epitope Target: Stat4

Epitope Species: mouse

Product Reactivity/Detection: Stat4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Stat4 Antibody (C-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-Stat4 antibody is p-Stat4 Antibody (E-2): sc-28296.