p-Stat4 Antibody (E-2): sc-28296

p-Stat4 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 721 phosphorylated Stat4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Stat4 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Stat4 plays a crucial role in the immune response, particularly in mediating signals from interleukin-12 (IL-12), which is essential for the differentiation of T helper 1 (Th1) cells. Upon activation by IL-12, Stat4 undergoes phosphorylation, leading to dimerization and subsequent translocation to the nucleus, where Stat4 binds to specific DNA elements to regulate gene expression. Phosphorylation of Ser 721 is particularly important as this modification is necessary for transcriptional activity of Stat4 and depends on p38 MAPK signaling, highlighting intricate regulatory mechanisms that control immune responses. anti-Stat4 antibody (E-2) specifically detects this phosphorylated form, making p-Stat4 (E-2) monoclonal antibody an invaluable tool for researchers studying immune signaling pathways and Stat4′s role in various biological processes.

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p-Stat4 Antibody (E-2) References:

1. Cytosolic tyrosine dephosphorylation of STAT5. Potential role of SHP-2 in STAT5 regulation.  |  Yu, CL., et al. 2000. J Biol Chem. 275: 599-604. PMID: 10617656
2. IL-12 selectively regulates STAT4 via phosphatidylinositol 3-kinase and Ras-independent signal transduction pathways.  |  Athié-M, V., et al. 2000. Eur J Immunol. 30: 1425-34. PMID: 10820390
3. Serine phosphorylation of STATs.  |  Decker, T. and Kovarik, P. 2000. Oncogene. 19: 2628-37. PMID: 10851062
4. Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity.  |  Visconti, R., et al. 2000. Blood. 96: 1844-52. PMID: 10961885
5. Detection of intracellular phosphorylated STAT-4 by flow cytometry.  |  Uzel, G., et al. 2001. Clin Immunol. 100: 270-6. PMID: 11513540
6. Constitutive tyrosine and serine phosphorylation of STAT4 in T-cells transformed with HTLV-I.  |  Higashi, T., et al. 2005. Genes Cells. 10: 1153-62. PMID: 16324152
7. The psoriasis-protective TYK2 I684S variant impairs IL-12 stimulated pSTAT4 response in skin-homing CD4+ and CD8+ memory T-cells.  |  Enerbäck, C., et al. 2018. Sci Rep. 8: 7043. PMID: 29728633
8. STAT4 Phosphorylation of T-helper Cells predicts surgical outcomes in Refractory Chronic Rhinosinusitis.  |  Abidin, MR., et al. 2023. medRxiv.. PMID: 38168423
9. Downregulated GPX4 in salivary gland epithelial cells contributes to salivary secretion dysfunction in Sjogren's syndrome via lipid ROS/pSTAT4/AQP5 axis.  |  Zhou, J., et al. 2024. Free Radic Biol Med. 218: 1-15. PMID: 38574973
10. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
11. Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation.  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
12. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Stat4 Antibody (E-2) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Stat4 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Stat4 Antibody (E-2) Specifications

Alternate Product Name: Stat4

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 721

Epitope Target: Stat4

Epitope Species: human

Product Reactivity/Detection: Ser 721 phosphorylated Stat4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for p-Stat4 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent