SPTLC1 Antibody (H-1): sc-374143

SPTLC1 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPTLC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPTLC1 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPTLC1, also known as serine palmitoyltransferase 1 or LCB1, plays a crucial role in sphingolipid biosynthesis by catalyzing the conversion of L-serine and palmitoyl-CoA into 3-oxosphinganine, a key precursor in the synthesis of complex sphingolipids. This process is vital for maintaining cellular membrane integrity and signaling, as sphingolipids are essential components of cell membranes and are involved in various cellular functions, including apoptosis, cell growth, and differentiation. SPTLC1 activity is regulated by the availability of substrates and cofactors, and any dysregulation can lead to significant health issues, such as hereditary sensory neuropathy, atopic eczema, and psoriasis, highlighting SPTLC1′s importance in human health.

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SPTLC1 Antibody (H-1) References:

1. Increased expression of serine palmitoyltransferase (SPT) in balloon-injured rat carotid artery.  |  Uhlinger, DJ., et al. 2001. Thromb Haemost. 86: 1320-6. PMID: 11816724
2. Permeability barrier disruption increases the level of serine palmitoyltransferase in human epidermis.  |  Stachowitz, S., et al. 2002. J Invest Dermatol. 119: 1048-52. PMID: 12445191
3. Characterization of serine palmitoyltransferase in normal human tissues.  |  Batheja, AD., et al. 2003. J Histochem Cytochem. 51: 687-96. PMID: 12704216
4. Enhanced serine palmitoyltransferase expression in proliferating fibroblasts, transformed cell lines, and human tumors.  |  Carton, JM., et al. 2003. J Histochem Cytochem. 51: 715-26. PMID: 12754283
5. Activity of partially inhibited serine palmitoyltransferase is sufficient for normal sphingolipid metabolism and viability of HSN1 patient cells.  |  Dedov, VN., et al. 2004. Biochim Biophys Acta. 1688: 168-75. PMID: 14990347
6. Mutant SPTLC1 dominantly inhibits serine palmitoyltransferase activity in vivo and confers an age-dependent neuropathy.  |  McCampbell, A., et al. 2005. Hum Mol Genet. 14: 3507-21. PMID: 16210380
7. Mutations in the SPTLC1 protein cause mitochondrial structural abnormalities and endoplasmic reticulum stress in lymphoblasts.  |  Myers, SJ., et al. 2014. DNA Cell Biol. 33: 399-407. PMID: 24673574
8. New Insights into the Neuromyogenic Spectrum of a Gain of Function Mutation in SPTLC1.  |  Kölbel, H., et al. 2022. Genes (Basel). 13: PMID: 35627278
9. Pathogenic variants in the SPTLC1 gene cause hyperkeratosis lenticularis perstans.  |  Jägle, S., et al. 2023. Br J Dermatol. 188: 94-99. PMID: 36689507
10. Heterozygous SPTLC1 p.Leu39del is a major cause of slow-progressing juvenile ALS.  |  Guissart, C., et al. 2024. J Neurol Neurosurg Psychiatry. 95: 288-290. PMID: 38041669
11. Effect of SPTLC1 on type 2 diabetes mellitus.  |  Yi, B., et al. 2025. World J Diabetes. 16: 94861. PMID: 39959268
12. Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis.  |  Weiss, B. and Stoffel, W. 1997. Eur J Biochem. 249: 239-47. PMID: 9363775

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SPTLC1 Antibody (H-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-SPTLC1 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SPTLC1 Antibody (H-1) Specifications

Alternate Product Name: SPTLC1

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 174-473

Epitope Target: SPTLC1

Epitope Species: human

Product Reactivity/Detection: SPTLC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for SPTLC1 Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin