SPT7L Antibody (F-5): sc-514548
- SPT7L Antibody (F-5) is a mouse monoclonal IgG2b κ SPT7L antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 182-414 mapping at the C-terminus of SPT7L of human origin
- SPT7L Antibody (F-5) is recommended for detection of SPT7L of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SPT7L Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPT7L Antibody (F-5) for WB applications. These reagents are now offered in bundles with SPT7L Antibody (F-5) (see ordering information below).
SPT7L Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects SPT7L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SPT7L antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPT7L protein, also known as suppressor of Ty 7-like or STAGA complex 65 subunit gamma, is a 414 amino acid sumoylated nuclear protein that plays a crucial role in transcriptional regulation as a component of the STAGA transcription coactivator-HAT complex. This complex is essential for the acetylation of histones, which is a key modification that facilitates the transcription of genes by altering chromatin structure. Proper functioning of SPT7L is vital for gene expression and cellular processes, and dysregulation has been implicated in various cancers, including adenocarcinomas and gliomas, where SPT7L is highly expressed. The gene encoding SPT7L is located on human chromosome 2, which is notable for its size and the number of genes encoded, as well as its association with several genetic diseases. Understanding the role of SPT7L and interactions within the STAGA complex can provide insights into the mechanisms of transcriptional regulation and potential therapeutic targets for cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPT7L Antibody (F-5) References:
- A new tumor-rejection antigen recognized by cytotoxic T lymphocytes infiltrating into a lung adenocarcinoma. | Nishizaka, S., et al. 2000. Cancer Res. 60: 4830-7. PMID: 10987294
- Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. | Martinez, E., et al. 2001. Mol Cell Biol. 21: 6782-95. PMID: 11564863
- Recognition of a new ARTC1 peptide ligand uniquely expressed in tumor cells by antigen-specific CD4+ regulatory T cells. | Wang, HY., et al. 2005. J Immunol. 174: 2661-70. PMID: 15728473
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. | Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
- The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners. | Soutoglou, E., et al. 2005. Mol Cell Biol. 25: 4092-104. PMID: 15870280
- Evidence for an ancestral alphoid domain on the long arm of human chromosome 2. | Avarello, R., et al. 1992. Hum Genet. 89: 247-9. PMID: 1587535
- Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. | Jeronimo, C., et al. 2007. Mol Cell. 27: 262-274. PMID: 17643375
- Multivalent binding of p53 to the STAGA complex mediates coactivator recruitment after UV damage. | Gamper, AM. and Roeder, RG. 2008. Mol Cell Biol. 28: 2517-27. PMID: 18250150
- Origin of human chromosome 2: an ancestral telomere-telomere fusion. | IJdo, JW., et al. 1991. Proc Natl Acad Sci U S A. 88: 9051-5. PMID: 1924367
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT7L Antibody (F-5) | sc-514548 | 200 µg/ml | $322.00 | |||
SPT7L Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-524763 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPT7L Antibody (F-5): m-IgG2b BP-HRP Bundle | sc-549180 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPT7L Antibody (F-5) AC | sc-514548 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPT7L Antibody (F-5) HRP | sc-514548 HRP | 200 µg/ml | $322.00 | |||
SPT7L Antibody (F-5) FITC | sc-514548 FITC | 200 µg/ml | $336.00 | |||
SPT7L Antibody (F-5) PE | sc-514548 PE | 200 µg/ml | $349.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 488 | sc-514548 AF488 | 200 µg/ml | $364.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 546 | sc-514548 AF546 | 200 µg/ml | $364.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 594 | sc-514548 AF594 | 200 µg/ml | $364.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 647 | sc-514548 AF647 | 200 µg/ml | $364.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 680 | sc-514548 AF680 | 200 µg/ml | $364.00 | |||
SPT7L Antibody (F-5) Alexa Fluor® 790 | sc-514548 AF790 | 200 µg/ml | $364.00 |