Spt3 Antibody (C-1): sc-393567
- Spt3 Antibody (C-1) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 92-113 within an internal region of Spt3 of Saccharomyces cerevisiae origin
- recommended for detection of Spt3 of y origin by WB, IP, IF and ELISA
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Spt3 Antibody (C-1) for WB applications. These reagents are now offered in bundles with Spt3 Antibody (C-1) (see ordering information below).
Spt3 Antibody (C-1) is a mouse monoclonal IgG2b light chain antibody that detects Spt3 in yeast by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spt3 plays a crucial role in the transcriptional regulation of RNA polymerase II-dependent genes, particularly as a member of the SAGA complex in Saccharomyces cerevisiae. Spt3 is essential for recruiting the TATA-box binding protein (TBP) to core promoters, facilitating the assembly of the transcription machinery necessary for gene expression. Notably, Spt3′s function is intricately linked to modulating transcriptional activity; while Spt3 can inhibit TBP binding at certain promoters, leading to reduced transcription of genes such as HO, HIS3, and TRP3, GCN5, another SAGA complex member, can counteract this inhibition by enhancing TBP/TFIIA complex formation through histone acetylation. The dynamic interplay between Spt3 and other transcription factors underscores Spt3′s importance in regulating mating and developmental pathways in yeast, making anti-Spt3 antibody (C-1) a valuable tool for researchers studying gene expression and regulatory mechanisms in this model organism.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spt3 Antibody (C-1) | sc-393567 | 200 µg/ml | $322.00 | |||
Spt3 Antibody (C-1): m-IgGκ BP-HRP Bundle | sc-535824 | 200 µg Ab; 40 µg BP | $361.00 | |||
Spt3 Antibody (C-1): m-IgG2b BP-HRP Bundle | sc-549482 | 200 µg Ab; 10 µg BP | $361.00 | |||
Spt3 (C-1) Neutralizing Peptide | sc-393567 P | 100 µg/0.5 ml | $69.00 |