Spot 14 Antibody (D-2): sc-390953

Spot 14 Antibody (D-2) is a mouse monoclonal IgG1 antibody that detects Spot 14 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spot 14, also known as S14 or THRSP (thyroid hormone responsive protein), is a small acidic protein primarily localized in the nucleus, playing a crucial role in tissues that synthesize triglycerides, including the liver, mammary glands, and adipose tissues. Spot 14 (D-2) antibody targets a protein essential for growth and differentiation, functioning as a transcriptional regulator for genes involved in lipogenesis, which is the metabolic process of synthesizing fatty acids. Spot 14 expression is activated by various lipogenic stimuli, such as thyroid hormone, dietary carbohydrates, insulin, and glucose, while being downregulated by catecholamines and glucagon. Spot 14 expression exhibits oscillation in accordance with the circadian clock, highlighting its potential role in metabolic regulation throughout the day. Knockdown of Spot 14 has been shown to impair lipid synthesis and induce apoptosis, indicating its vital function in cellular metabolism. In breast cancer, Spot 14 is often overexpressed, suggesting enhanced cell growth and survival, making anti-Spot 14 antibody (D-2) a significant tool for cancer biology research.

The preferred Spot 14 antibody is Spot 14 Antibody (F-7): sc-137178.