Spir-1 Antibody (H-1): sc-515448
- Spir-1 Antibody (H-1) is a mouse monoclonal IgG1 κ Spir-1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 411-557 mapping within an internal region of Spir-1 of human origin
- Spir-1 Antibody (H-1) is recommended for detection of Spir-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Spir-1 Antibody (H-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Spir-1 Antibody (H-1) for WB applications. These reagents are now offered in bundles with Spir-1 Antibody (H-1) (see ordering information below).
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Spir-1 Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Spir-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Spir-1 (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spir-1, also known as spire homolog 1, is a 756 amino acid protein that plays a crucial role as an actin nucleation factor, facilitating new actin filament growth and participating in vesicle transport processes. Spir-1 localization to the cytoskeleton and perinuclear region of the cytoplasm is vital for maintaining cellular structure and function, effectively linking intracellular transport mechanisms with actin organization, which is essential for various cellular activities, including cell motility and division. One KIND domain and two WH2 domains in Spir-1 contribute to actin interaction, further underscoring Spir-1′s importance in cytoskeletal dynamics. Multiple isoforms of Spir-1 arise from alternative splicing, allowing for functional diversity in different cellular contexts. Spir-1 encoding gene is located on human chromosome 18, a region associated with several genetic disorders, including Trisomy 18, Niemann-Pick disease, and hereditary hemorrhagic telangiectasia, highlighting Spir-1′s significance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Spir-1 Antibody (H-1) References:
- Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. | Hirosawa, M., et al. 1999. DNA Res. 6: 329-36. PMID: 10574461
- The Spir actin organizers are involved in vesicle transport processes. | Kerkhoff, E., et al. 2001. Curr Biol. 11: 1963-8. PMID: 11747823
- Overlapping expression pattern of the actin organizers Spir-1 and formin-2 in the developing mouse nervous system and the adult brain. | Schumacher, N., et al. 2004. Gene Expr Patterns. 4: 249-55. PMID: 15053972
- Drosophila Spire is an actin nucleation factor. | Quinlan, ME., et al. 2005. Nature. 433: 382-8. PMID: 15674283
- Targeted proteomic analysis of 14-3-3 sigma, a p53 effector commonly silenced in cancer. | Benzinger, A., et al. 2005. Mol Cell Proteomics. 4: 785-95. PMID: 15778465
- Analysis of the function of Spire in actin assembly and its synergy with formin and profilin. | Bosch, M., et al. 2007. Mol Cell. 28: 555-68. PMID: 18042452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Spir-1 Antibody (H-1) | sc-515448 | 200 µg/ml | $322.00 | |||
Spir-1 Antibody (H-1): m-IgG Fc BP-HRP Bundle | sc-531373 | 200 µg Ab; 10 µg BP | $361.00 | |||
Spir-1 Antibody (H-1): m-IgGκ BP-HRP Bundle | sc-525047 | 200 µg Ab, 40 µg BP | $361.00 | |||
Spir-1 Antibody (H-1): m-IgG1 BP-HRP Bundle | sc-544512 | 200 µg Ab; 20 µg BP | $361.00 | |||
Spir-1 Antibody (H-1) AC | sc-515448 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Spir-1 Antibody (H-1) HRP | sc-515448 HRP | 200 µg/ml | $322.00 | |||
Spir-1 Antibody (H-1) FITC | sc-515448 FITC | 200 µg/ml | $336.00 | |||
Spir-1 Antibody (H-1) PE | sc-515448 PE | 200 µg/ml | $349.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 488 | sc-515448 AF488 | 200 µg/ml | $364.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 546 | sc-515448 AF546 | 200 µg/ml | $364.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 594 | sc-515448 AF594 | 200 µg/ml | $364.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 647 | sc-515448 AF647 | 200 µg/ml | $364.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 680 | sc-515448 AF680 | 200 µg/ml | $364.00 | |||
Spir-1 Antibody (H-1) Alexa Fluor® 790 | sc-515448 AF790 | 200 µg/ml | $364.00 |