Anti-SphK1 Antibody (G-11) recommended for detection of SphK1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-SphK1 Antibody (G-11): sc-365401

Anti-SphK1 Antibody (G-11) is rated 4.3 out of 5 by 8.
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Datasheets

    • Anti-SphK1 Antibody (G-11) is a mouse monoclonal IgG1 κ SphK1 antibody, cited in 4 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 331-351 near the C-terminus of SphK1 of mouse origin
    • Anti-SphK1 Antibody (G-11) is recommended for detection of SphK1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SphK1 Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SphK1 (G-11): sc-365401.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for SphK1 Antibody (G-11) for WB applications. This reagent is now offered in a bundle with SphK1 Antibody (G-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-365401 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Good antibody for Western blot Produced positive Western blot data of SphK1 expression in mouse mesenteric arteries extracts.
Date published: 2022-02-09
Rated 5 out of 5 by from Works great Tried the SphK1 antibody for looking at expression in mesenteric arteries using western blot. Validated the bands with knockdown using siRNA.
Date published: 2020-07-22
Rated 5 out of 5 by from Great... Ab works excellent... Thank you Santacruz. The sample of SphK1 Ab you have supplied was awsome. Good results. :-)
Date published: 2018-02-12
Rated 4 out of 5 by from Specific for WB Tested on SPHK1 K.O. together with control on Brain tissue. Showed no bands in the mutant. However, a bit dirty.
Date published: 2018-01-04
Rated 1 out of 5 by from Does not recognize SphK1 While this antibody recognizes a band in the correct MW range of SphK1, it does not appear to actually be specific for SphK1. We tested the antibody by western blotting using wild type and SphK1 KO knockout cell lysate as well as with recombinant crystallography grade SphK1 as a positive control. Both cell lysates were immunopositive, but the positive control was not. If you would like me to upload images please let me know. We did receive this antibody as a free sample, and our lab has extensive experience with this protein.
Date published: 2017-12-04
Rated 4 out of 5 by from Working well SphK1 antibody in WB This SphK1 antibody works well in WB with human liver and mouse liver, kidney and brain.
Date published: 2017-10-12
Rated 5 out of 5 by from Potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium-strong detection of the ~42 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced nice Western blot data of SphK1 Produced nice Western blot data of SphK1 expression in PC-12 whole cell lysate. -SCBT QC
Date published: 2013-06-15
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