SPFH1 Antibody (A-7): sc-514820
- SPFH1 Antibody (A-7) is a mouse monoclonal IgG1 κ SPFH1 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 250-277 within an internal region of SPFH1 of human origin
- SPFH1 Antibody (A-7) is recommended for detection of SPFH1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SPFH1 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SPFH1 Antibody (A-7) for WB applications. These reagents are now offered in bundles with SPFH1 Antibody (A-7) (see ordering information below).
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SPFH1 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPFH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPFH1 (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPFH1, also known as stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1 or ERLIN1, is a 346 amino acid protein located in the endoplasmic reticulum (ER) membrane and is a member of the band 7/mec-2 family. SPFH1 plays a crucial role in forming a heteromeric complex with SPFH2, which is essential for mediating the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). The SPFH1/SPFH2 complex is characterized by a ring-shaped structure, vital for maintaining cellular homeostasis and regulating calcium signaling pathways. SPFH1 is predominantly expressed in various tissues, including heart, placenta, liver, kidney, pancreas, prostate, testis, ovary, and small intestine, highlighting its importance in diverse physiological processes. The SPFH1 gene is located on human chromosome 10q21-q22, which encompasses over 131 million base pairs and contains a significant number of protein-coding genes, underscoring the complexity and significance of this genomic region in human health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPFH1 Antibody (A-7) References:
- Identification and characterization of a novel gene KE04 differentially expressed by activated human dendritic cells. | Li, N., et al. 2000. Biochem Biophys Res Commun. 279: 487-93. PMID: 11118313
- The DNA sequence and comparative analysis of human chromosome 10. | Deloukas, P., et al. 2004. Nature. 429: 375-81. PMID: 15164054
- An endoplasmic reticulum (ER) membrane complex composed of SPFH1 and SPFH2 mediates the ER-associated degradation of inositol 1,4,5-trisphosphate receptors. | Pearce, MM., et al. 2009. J Biol Chem. 284: 10433-45. PMID: 19240031
- Substrate-specific mediators of ER associated degradation (ERAD). | Brodsky, JL. and Wojcikiewicz, RJ. 2009. Curr Opin Cell Biol. 21: 516-21. PMID: 19443192
- When worlds collide: IP(3) receptors and the ERAD pathway. | Wojcikiewicz, RJ., et al. 2009. Cell Calcium. 46: 147-53. PMID: 19709743
- SPFH1 and SPFH2 mediate the ubiquitination and degradation of inositol 1,4,5-trisphosphate receptors in muscarinic receptor-expressing HeLa cells. | Wang, Y., et al. 2009. Biochim Biophys Acta. 1793: 1710-8. PMID: 19751772
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPFH1 Antibody (A-7) | sc-514820 | 200 µg/ml | $322.00 | |||
SPFH1 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-531226 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPFH1 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-524853 | 200 µg Ab, 40 µg BP | $361.00 | |||
SPFH1 Antibody (A-7): m-IgG1 BP-HRP Bundle | sc-544442 | 200 µg Ab; 20 µg BP | $361.00 | |||
SPFH1 Antibody (A-7) AC | sc-514820 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPFH1 Antibody (A-7) HRP | sc-514820 HRP | 200 µg/ml | $322.00 | |||
SPFH1 Antibody (A-7) FITC | sc-514820 FITC | 200 µg/ml | $336.00 | |||
SPFH1 Antibody (A-7) PE | sc-514820 PE | 200 µg/ml | $349.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 488 | sc-514820 AF488 | 200 µg/ml | $364.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 546 | sc-514820 AF546 | 200 µg/ml | $364.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 594 | sc-514820 AF594 | 200 µg/ml | $364.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 647 | sc-514820 AF647 | 200 µg/ml | $364.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 680 | sc-514820 AF680 | 200 µg/ml | $364.00 | |||
SPFH1 Antibody (A-7) Alexa Fluor® 790 | sc-514820 AF790 | 200 µg/ml | $364.00 | |||
SPFH1 (A-7) Neutralizing Peptide | sc-514820 P | 100 µg/0.5 ml | $69.00 |